NHLF – Human Lung Fibroblasts
NHLF – Human Lung Fibroblasts
Cryopreserved ampule of Normal Human Lung Fibroblasts (NHLF) containing ≥ 500,000 cells
Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research
Product Overview
Lonza's Human Lung Fibroblasts are isolated from adult lung tissue. Some matched donor sets of lung fibroblasts, bronchial smooth muscle cells, bronchial epithelial cells may be available. Contact Customer Service or Scientific Support for more information.
Lung fibroblasts play a role in airway inflammation and airway remodeling. They can be a target for asthma medications in pharmaceutical development. The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, for example, they have long branching processes and gap junctions. Their principle function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa.
Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. Important research applications includ pulmonary disease, i.e. COPD, asthma, CF, TB, cancer.
Cryopreserved CloneticsTM Normal Human Lung Fibroblasts cells are guaranteed through 15 population doublings, they are tested for negative von Willebrand Factor Expression/Factor VIII, cytokeratins 18 and 19, and alpha smooth muscle actin All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Purchase our Lung Fibroblast Cell Culture Kit (catalog no. CC-2512B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of lung fibroblast cells (catalog no. CC-2512) and FGMTM-2 BulletKitTM Growth Media (catalog no. CC-3132)
Request More InfoStorage and Content
1 x Cryopreserved ampule of NHLF containing ≥ 500,000 cells
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
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TechSheet - Lung Fibroblast Cell Systems
Technical information sheet for Clonetics™ Lung Fibroblast cells -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Instructions - Human Fibroblast Cell Systems
Technical Instructions for culturing Clonetics™ Human Fibroblasts Cells
Educational Material
Brochures, White Papers etc.
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Asthma- and COPD-related Differential Gene Expression in Primary Human Lung Fibroblasts
A white paper exploring differential gene expression in primary human lung fibroblasts -
Primary Human Airway Cells and Media
Airway Cell and Media Reference Guide -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Primary Cells for Airway Research – Infographic
Infographic showing how Lonza primary cells can aide in your airway research -
Supporting In Vitro ADMETox with an Advanced Portfolio of Primary Cells and Media
Reference to Help Get Started on Primary Cell Culture for ADMETox Applications
NHLF – Human Lung Fibroblasts
Immunohistochemistry staining of normal human lung fibroblasts (CC-2512) stained with primary antibody Anti-Vimentin AB24525 (AbCam) used at 1:5000 dilution. Secondary antibody used was Horse anti Mouse, DI 2488 VectorLabs, used at 1:200 dilution. Nuclei were stained with Hoechst at 1:10,000.
NHLF – Human Lung Fibroblasts
Immunohistochemistry staining of normal human lung fibroblasts (CC-2512) stained with primary antibody Anti-Vimentin AB24525 (AbCam) used at 1:5000 dilution. Secondary antibody used was , Goat anti Chicken Alexa 594, A11042 Invitrogen: Used at 1:200, Horse anti Mouse, DI 2488 VectorLabs: Used at 1:200 Nuclei were stained with Hoechst.