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SG Cell Line 4D-Nucleofector™ X Kit S

SG Cell Line 4D-Nucleofector™ X Kit S

Catalog #: V4XC-3032
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (20 µL format).
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Product Overview

The SG Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit.

This kit has been determined to be optimal for transfecting following cell types:

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection® conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

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Benefits

  • High transfection efficiencies in difficult-to-transfect cell lines
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-Nucleofector® System, 96-well ShuttleTM System and 384-well Nucleofector® System

Applications

  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
  • Medium throughput applications
  • Optimization of transfection conditions, e.g. substrate titrations

Storage and Content

1 x 0.675 mL SG Cell Line Nucleofector® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μl)

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Raji Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of Raji cells with the SG Cell Line 4D-Nucleofector™ X Kit
  • Ramos Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of Ramos cells with the SG Cell Line 4D-Nucleofector™ X Kit
  • THP-1 Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of THP-1 cells with the SG Cell Line 4D-Nucleofector™ X Kit
  • CHO-S [Suspension] Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of CHO-S cells (Invitrogen) with the SG Cell Line 4D-Nucleofector™ X Kit
  • NIH/3T3 Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of NIH/3T3 cells with the SG Cell Line 4D-Nucleofector™ X Kit
  • BA/F3 Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of BA/F3 cells (DSMZ) with the SG Cell Line 4D-Nucleofector™ X Kit
  • H4 Transfection Protocol - 4D-Nucleofector™ X-Unit

    Instructions of use for transfection of H4 cells with the SG Cell Line 4D-Nucleofector™ X Kit
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