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OsteoImage™ Mineralization Assay, 500 Test

OsteoImage™ Mineralization Assay, 500 Test

Catalog #: PA-1503

OsteoImageTM Mineralization Assay is a rapid, fluorescent in vitro assay for assessing bone cell mineralization, 500 tests. 

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 727,00 EUR
EA

Product Overview

The OsteoImageTM Mineralization Assay is a rapid, fluorescent in vitro assay for assessing bone cell mineralization. The OsteoImageTM Assay can quantitate in vitro mineralization by osteogenic stem cells, primary osteoblasts, and osteoblast-like cell lines. It is based on specific binding of the fluorescent OsteoImageTM Staining Reagent to the hydroxyapatite portion of bone-like nodules deposited by cells. 

Unlike typical histochemical methods such as von Kossa and Alizarin red, neither of which is hydroxyapatite specific, the OsteoImageTM Assay eliminates multiple steps or tedious extraction steps.

Increase the speed, sensitivity and ease of measuring mineralization in your cell cultures with the OsteoImageTM Mineralization Assay.

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Benefits

  • Delivers qualitative visual fluorescent microscopy or quantitative fluorescent plate reader results
  • Can be used with primary osteoblasts, osteoblast stem cells, and osteoblast cell lines
  • Measures hydroxyapatite, similar to real bone
  • Completed in less than 90 minutes, without tedious extractions
  • Sensitive enough to detect time dependent increases in mineralization in differentiating cells
  • Scalable for use in 6-well to 96-well plates

Storage and Content

  • 5 x 96 well plates
  • 1 x 500 μl OsteoImageTM Staining Reagent
  • 1 x 50 mL Staining Reagent Dilution Buffer
  • 1 x 50 mL OsteoImageTM Wash Buffer (10x)
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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • TechSheet - Rat Calvariae Osteoblast Cell System

    Technical tips for culturing Rat Calvariae Osteoblasts
  • Instructions - OsteoImage™ Mineralization Assay

    Instructions for proper use of the OsteoImage™ Mineralization Assay
  • Instructions - Poietics™ ADSC Osteogenesis Differentiation

    Instructions for Proper Use
  • Instructions - Rat Calvariae Osteoblast Cell System

    Instructions for proper use and culture of Rat Calvariae Osteoblasts
  • TechSheet – Rat Calvariae Osteoblast Cell System (R-Ost-583)

    Technical tips for culturing Rat Calvariae Osteoblast Cells
  • Stem Cell Research Tools for Biologically Relevant Results

    Learn how Lonza products can aide in your stem cell research
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Educational Material

Brochures, White Papers etc.

  • OsteoImage™ Mineralization Assay

    Informational application notes for OsteoImage™ Mineralization Assay
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Data

OsteoImage™ Mineralization Assay Differentiation Data

Differentiation Data for Various Cell Types Using the OsteoImage™ Mineralization Assay

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