PYROGENT™-5000 Bulk Kinetic Turbidimetric LAL Assay, 4500 Test Kit
PYROGENT™-5000 Bulk Kinetic Turbidimetric LAL Assay, 4500 Test Kit
Product Overview
The PYROGENTTM–5000 Kinetic Turbidimetric LAL Assay is a quantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent, placed in the incubating absorbance plate reader, and automatically monitored over time (via WinKQCLTM Software) for the appearance of turbidity. In the presence of endotoxin, the lysate will begin to gel, causing the solution to become cloudy or turbid. The time required for the change is inversely proportional to the amount of endotoxin present. The concentration in unknown samples can be calculated from a standard curve. The PYROGENTTM–5000 Kinetic Turbidimetric Assay is perfect for laboratories needing to process large numbers of samples. This assay is performed in a 96-well plate allowing many samples to be processed at one time. It is ideal for water samples, large volume parenterals and water rinse from medical devices. The PYROGENTTM–5000 Kinetic Turbidimetric Assay is measured at the 340 nm wavelength and has a sensitivity range from 0.01 to 100.0 EU/ml. PYROGENTTM-5000 Bulk Kinetic Turbidimetric LAL Assay, N688, is made to order and requires a lead time. Please contact your local sales representative for further information.
Request More InfoBenefits
- Sensitivity range from 0.01 to 100 EU/ml
Applications
- Cost-effective method for water and large volume parenterals testing
Storage and Content
- 45 x 100 tests/vial lysate
- 45 vials reconstitution buffer
- 10 vials endotoxin
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
Educational Material
Brochures, White Papers etc.
Videos
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Troubleshooting Guide - Kinetic LAL Assay Procedure (English)
A step-by-step guide depicting how to perform a traditional kinetic LAL assay. -
Troubleshooting Guide - Testing Oil Solutions with the PYROGENT™-5000 and Kinetic-QCL™ Assays
Oil-based parenterals have always been a challenge in endotoxin detection assays. This Tech Tip describes a liquid-liquid extraction procedure that can be used to remove the endotoxin from the oil into an aqueous phase, which can then be tested with the PYROGENT™-5000 or Kinetic-QCL™ Assays. -
Technical Tip - Serum, Plasma Testing with PYROGENT® 5000 and Kinetic-QCL® Assays
The article focuses on the recommended procedure that can be used to prepare serum or plasma for LAL-based endotoxin testing.
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How To Perform The PYROGENT™-5000 LAL Assasy
The PYROGENT™-5000 Kinetic Turbidimetric LAL Assay is a quantitative; kinetic assay for the detection of Gram-negative bacterial endotoxin. In this assay demonstration; we show how to perform a routine test that could be used for product release.