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Senescent Zombie in Primary Cell Culture

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Article overview

The effect of light on media performance

Light exposure causes photoxidation which can generate harmful, highly reactive species that are damaging to your culture.

Tech tips for primary cell culture: Common errors

Cell culture is expensive and time consuming. Effective technique and protocols can help keep cells healthy in culture. These are some common errors to avoid when culturing primary cells. 

Quick tips for successful Nucleofection® experiments

Sometimes it’s the basics that can make or break an experiment and Nucleofection® experiments are no different. Cell handling, proper culture conditions, and following the protocol are all critical. Here are some important things to keep in mind when performing your own Nucleofection® experiments.

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References

King A. Zombie cells hold clues to COPD progression. Nature. 2020 May 13

Kumari R, Jat P. Mechanisms of Cellular Senescence: Cell Cycle Arrest and Senescence Associated Secretory Phenotype. Front Cell Dev Biol. 2021 Mar 29

Liao CM, Wulfmeyer VC, Chen R, Erlangga Z, Sinning J, von Mässenhausen A, Sörensen-Zender I, Beer K, von Vietinghoff S, Haller H, Linkermann A, Melk A, Schmitt R. Induction of ferroptosis selectively eliminates senescent tubular cells. Am J Transplant. 2022 May 23