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Product Overview
SeaPlaque® Agarose is the original low melting temperature agarose. This molecular biology grade agarose produces gels with greater sieving properties and higher clarity than standard melting temperature agarose. The low melting temperature of SeaPlaque® Agarose makes it ideal for preparative DNA and RNA electrophoresis, while its low gelling temperature is ideal for cloning of tissue culture cells and viral plaque assays.
Analytical Specifications
Gelling temperature (1.5%) 26°C - 30°C
Melting temperature (1.5%) ≤65°C
Gel strength (1%) ≥200 g/cm²
Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Request More InfoBenefits
- All Lonza Agarose is quality tested to certify performance
- Low EEO
- Low gelling and melting temperature
Applications
- Cloning of tissue culture cells,
- Viral plaque assays,
- DNA/RNA preparative electrophoresis
Storage and Content
- SeaPlaque® Agarose 25 g
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
Educational Material
Brochures, White Papers etc.
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SourceBook Section VIII - Separation of RNA in Agarose Gels
1. Introduction 2. Preparation of RNA Samples 3. Buffers for RNA Electrophoresis 4. Electrophoresis of RNA 5. Detection of RNA in Agarose Gels with GelStar® or SYBR® Green II Gel Stains 6. Detecting RNA with Ethidium Bromide 7. Northern Blotting 8. FlashGel® System for RNA Analysis 9. References -
SourceBook Section II - Preparation of Agarose Gels
1. Agarose Selection Guide 2. Agarose and Compatible Techniques 3. Agarose Types 4. Agarose Analytical Specifications 5. Suggested Agarose Concentrations & Dye Migration Information 6. Buffers for Electrophoresis 7. Dissolving Agarose 8. Casting Agarose Gels -
SourceBook Section IX - Special Applications in Agarose Gels
1. Amplification of Plasmid cDNA Libraries with SeaPrep® Agarose 2. Preparing Agarose for use in Cell Culture Applications 3. References -
Overview Lonza DNA Markers and Ladders
Select the best DNA Marker for your application -
SourceBook Section IV - Detection and Sizing of DNA in Agarose Gels
1. Guide to Lonza Ladders and Markers 2. Detecting DNA with GelStar® and SYBR® Green Nucleic Acid Gel Stains 3. Detecting DNA with Ethidium Bromide 4. High Sensitivity Detection using the FlashGel® System 5. References -
SourceBook Section III - Loading and Running DNA in Agarose Gels
1. DNA Loading 2. Loading Buffers 3. Optimal Voltage and Electrophoretic Times 4. Fast Running Protocols for High Resolution in MetaPhor® Agarose Gels 5. References -
SourceBook Section VI - Recovery of DNA from Agarose Gels
1. Tips for Increasing DNA Recovery Efficiency from Agarose Gels 2. b-Agarose Recovery of DNA from Agarose Gels 3. Electroelution of DNA from Agarose Gels 4. Phenol/Chloroform Extraction of DNA from Agarose Gels 5. "Modified Freeze/Squeeze" Extraction of DNA from Agarose Gels 6. Ethanol Precipitation of DNA Recovered from Agarose Gels 7. References -
Agarose Selection Guide
Select the best agarose for your application to minimize opportunity for error, optimize results and even reduce costs.
