SE Cell Line 4D-Nucleofector® X Kit L
SE Cell Line 4D-Nucleofector® X Kit L
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (100 µL format).
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Product Overview
The SE Cell Line 4D-Nucleofector® X Kit L is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette® Vessel format when working with the 4D-Nucleofector® X Unit.
This kit has been determined to be optimal for transfecting following cell types:
- 3T3-L1, diff., 3T3-L1, undiff.
- A-10, A7r5,
- BHK-21, BJ
- C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
- DU145
- GH3
- HCT116, HeLa, HeLa S3
- IMR-90
- Jurkat (DSMZ), Jurkat E6.1
- KG-1a, L6
- MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
- NCTC clone 929
- Panc-1
- T/G-HA VSMC, T-47D
- U2OS, U-87 MG
- WI-38
- T84
The respective cell type-specific Optimized Protocols are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 12 or 24 reactions.
Request More InfoBenefits
- High transfection efficiencies in difficult-to-transfect cell lines
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low throughput applications
Storage and Content
2 x 0.675 mL SE Cell Line Nucleofector® Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
12 x 100 µL Nucleocuvette® Vessel
12 x Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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4D-Nucleofector® Protocol for MRC-5
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MRC-5 -
4D-Nucleofector® Protocol for IMR90
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for IMR90 -
4D-Nucleofector® Protocol for Jurkatclone E6.1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Jurkatclone E6.1 -
4D-Nucleofector® Protocol for T-47D
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for T-47D -
4D-Nucleofector® Protocol for Calu-3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Calu-3 -
4D-Nucleofector® Protocol for A7r5
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A7r5 -
4D-Nucleofector® Protocol for MDA-MB-468
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MDA-MB-468 -
4D-Nucleofector® Protocol for HeLa cells
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HeLa cells -
4D-Nucleofector® Protocol for HCT116
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HCT116 -
4D-Nucleofector® Protocol for BJ
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for BJ
