Product Overview

Ready to use Rat Embryonic Dorsal Root Ganglion (DRG) Neurons are suspensions of high quality sensory neurons prepared by standardized methods, and are ready for immediate culture.

Each vial of Embryonic Dorsal Root Ganglion Neurons contains approximately 1 million cells in 0.25 ml suspension. Some cell death will occur during the first few days after plating and debris will be observed. This is normal. After approximately 4 days in culture, the cells will form a neurite network and by the 7th day, debris will be minimal.In the absence of mitotic inhibitors, the neurons tend to cluster (ganglionate) and detach from the substrate.

Rat Embryonic Dorsal Root Ganglion Neurons stain positive for Tuj. Primary neuronal cells need an appropriate substrate to adhere and survive. The preferred substrate is poly-D-lysine with laminin. Poly-D-lysine can also be used alone to coat the cell culture plasticware or cover slips. Coated cell culture plates, dishes, or cover slips can either be purchased from a supplier or prepared immediately prior to use.

Recommended medium: PNGM^TM BulletKit^TM with addtion of 100 ng/ml of Nerve Growth Factor-β from rat (Sigma No. N2513)*  

*IMPORTANT: For the survival and growth of embryonic DRG neurons, the addition of 100 ng/ml of Nerve Growth Factor to the media is required at all times (Sigma No. N2513). For inhibition of Schwann cell proliferation, mitotic inhibitors will also need to be added.