Primary Cell Optimization 4D-Nucleofector™ X Kit
Primary Cell Optimization 4D-Nucleofector™ X Kit
Product Overview
The Primary Cell Optimization 4D-NucleofectorTM X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-NucleofectorTM X Unit.
With the unique capability of the 4D-NucleofectorTM X Unit to address individual wells of a 16-well NucleocuvetteTM Strip with different programs, primary cell optimizations are easily performed within one experiment.
Complete Optimization: Using five 16-well NucleocuvetteTM Strips and our pmaxGFPTM Vector as positive control, our five Primary Cell NucleofectorTM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well NucleocuvetteTM Strip can be used for optional fine tuning or performing the first experiment with the selected conditions. Cell Group-Specific Optimization using
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell NucleofectorTM Solutions and NucleofectorTM Programs. Basic Protocols are available for following cell groups:
- Endothelial cells (mammalian)
- Epithelial cells (mammalian)
- Fibroblasts (mammalian)
- Glial cells (mammalian)
- Neurons (mammalian)
- Smooth muscle cells (mammalian)
- Stem cells (human)
Before doing a complete or cell group-specific optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
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Benefits
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined in the 16-well NucleocuvetteTM Strips are transferable to the 100 μL single NucleocuvetteTM in the 4D-NucleofectorTM X Unit
- Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
Applications
- Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-NucleofectorTM X Unit
Storage and Content
0.675 mL NucleofectorTM Solution P1
0.675 mL NucleofectorTM Solution P2
0.675 mL NucleofectorTM Solution P3
0.675 mL NucleofectorTM Solution P4
0.675 mL NucleofectorTM Solution P5
4 x 0.15 mL Supplement 1
1 x 0.15 mL Supplement 3
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
6 x 20 µL 16-well NucleocuvetteTM Strips
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
