Rat Brain Cortex Neurons
Rat Brain Cortex Neurons
Cryopreserved ampule of Rat Brain Cortex Neurons containing ≥4 million cells
Product Overview
Ready-to-use CloneticsTM Rat Brain Cortex Neurons from rat (E18, 19) brain are cell suspensions of high quality primary embryonic brain neuronal cells (including glia) prepared by standardized methods, and are ready for immediate culture.
Each vial of cortical cells contains approximately 4 million viable cells. The cells will seed into 10 or more wells of a 24-well plate and 50 or more wells of a 96-well plate using the recommended plating densities and medium.Cell death will occur during the first few days after plating and debris will be observed. This is normal. After approximately 4 days in culture, the cells will form a neurite network and by the 7th day, debris will be minimal.
Recommended Medium: PNGMTM BulletKitTM (CC-4461)
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Benefits
- Ready-To-Use
- No tissue dissection or animal handling required
- Increased data reproducibility
- Characteristic morphology and staining tests confirm tissue origin and cell type
- Negative for mycoplasma, bacteria, yeast and fungi
- Certificate of Analysis (CoA) is available for every batch
Applications
- Neurogenesis
- Neurotransmitter Function
- Gene Expression
- Signaling Pathways
- Electrophysiology
- Neurotoxicity
- Drug/Compound Screening
- Advanced Cell Culture Models
Storage and Content
1 x Cryopreserved ampule of Rat Cortex Neurons containing ≥ 4,000,000 cells
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Supplemental Inst. - Neuronal Plate Coating Instructions
Instructions of cell culture plate coating procedure required for successful neuronal culture -
Instructions - Rat Brain Cortex Neuronal Cell System
Instructions for proper use and culture of Rat Brain Cortex Neurons -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
TechSheet - Rat Brain Cortex Neuronal Cell System
Technical tips for culturing Rat Brain Cortex Neurons
Educational Material
Brochures, White Papers etc.
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Cryopreserved Neural Cells and Media
Lonza's Cryopreserved Neurons Offer Comparable Results to Fresh Cells -
Quality and Time: The Real Costs of Your Animal Neural Cell Isolation
Lonza infographic illustrating the benefits of using Lonza's Ready-to-Use Cells -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Adherent Nucleofection of Cryopreserved Dissociated Rat Brain Neuronal Cells
Results for transfecting cultured dissociated rat brain neurons (hippocampal or cortical) using the 4D-Nucleofector Y Unit. It compares results for freshly isolated neurons with Lonza cryopreserved neurons. -
Supporting In Vitro ADMETox with an Advanced Portfolio of Primary Cells and Media
Reference to Help Get Started on Primary Cell Culture for ADMETox Applications
