Product Overview
SeaPlaque® GTG® Agarose is a Genetic Technology Grade® Agarose used for separating DNA and PCR product fragments greater than 1,000 bp. This low melting temperature (65º C) agarose is ideally suited for direct enzymatic manipulation of nucleic acids in remelted agarose without additional DNA purification and is tested for the presence of proteases, ligases and nucleases. It is also compatible with PCR and sequencing reactions carried out in the presence of remelted agarose.
Analytical Specifications
Gelling temperature (1.5%) 26°C - 30°C
Melting temperature (1.5%) ≤65°C
Gel strength (1.0%) ≥200 g/cm²
Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Benefits
- All Lonza Agarose is quality tested to certify performance
- Low EEO
- Low melting temperature
- Tested for In-gel enzymatic reactions
- DNase/RNase Free
Applications
- Cloning of tissue culture cells
- Viral plaque assays
- DNA/RNA preparative electrophoresis
Storage and Content
- SeaPlaque® GTG® Agarose, 25 g
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
Educational Material
Brochures, White Papers etc.
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SourceBook Section VIII - Separation of RNA in Agarose Gels
1. Introduction 2. Preparation of RNA Samples 3. Buffers for RNA Electrophoresis 4. Electrophoresis of RNA 5. Detection of RNA in Agarose Gels with GelStar® or SYBR® Green II Gel Stains 6. Detecting RNA with Ethidium Bromide 7. Northern Blotting 8. FlashGel® System for RNA Analysis 9. References -
SourceBook Section II - Preparation of Agarose Gels
1. Agarose Selection Guide 2. Agarose and Compatible Techniques 3. Agarose Types 4. Agarose Analytical Specifications 5. Suggested Agarose Concentrations & Dye Migration Information 6. Buffers for Electrophoresis 7. Dissolving Agarose 8. Casting Agarose Gels -
SourceBook Section V - In-Gel Reactions
1. Overview 2. Tips for Increasing the Efficiency of In-Gel Reactions 3. Cloning in the Presence of Agarose 4. Restriction Digestion in the Presence of Agarose 5. DNA Amplification in the Presence of Agarose 6. References -
SourceBook Section IX - Special Applications in Agarose Gels
1. Amplification of Plasmid cDNA Libraries with SeaPrep® Agarose 2. Preparing Agarose for use in Cell Culture Applications 3. References -
Overview Lonza DNA Markers and Ladders
Select the best DNA Marker for your application -
SourceBook Section IV - Detection and Sizing of DNA in Agarose Gels
1. Guide to Lonza Ladders and Markers 2. Detecting DNA with GelStar® and SYBR® Green Nucleic Acid Gel Stains 3. Detecting DNA with Ethidium Bromide 4. High Sensitivity Detection using the FlashGel® System 5. References -
SourceBook Section III - Loading and Running DNA in Agarose Gels
1. DNA Loading 2. Loading Buffers 3. Optimal Voltage and Electrophoretic Times 4. Fast Running Protocols for High Resolution in MetaPhor® Agarose Gels 5. References -
SourceBook Section VI - Recovery of DNA from Agarose Gels
1. Tips for Increasing DNA Recovery Efficiency from Agarose Gels 2. b-Agarose Recovery of DNA from Agarose Gels 3. Electroelution of DNA from Agarose Gels 4. Phenol/Chloroform Extraction of DNA from Agarose Gels 5. "Modified Freeze/Squeeze" Extraction of DNA from Agarose Gels 6. Ethanol Precipitation of DNA Recovered from Agarose Gels 7. References -
Agarose Selection Guide
Select the best agarose for your application to minimize opportunity for error, optimize results and even reduce costs.
