Product Overview
SeaKem® GTGTM Agarose is a standard gelling temperature, high gel strength agarose that resolves DNA fragments greater than 100 bp. This Genetic Technology GradeTM (GTGTM) agarose is specifically designed for preparative DNA electrophoresis and is tested for the presence of proteases, ligases and nucleases. DNA recovered from SeaKem® GTGTM Agarose gels may be reliably digested and ligated. SeaKem® GTGTM Agarose is extensively performance tested to ensure complete compatibility with routine molecular biology techniques. A Certificate of Performance containing end-use test information is supplied with each shipment.
Analytical Specifications
Gelling temperature (1.5%) 36°C ±1.5°C
Melting temperature (1.5%) ≥90°C
Gel strength (1.5%) ≥1,200 g/cm²
Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Request More InfoBenefits
- All Lonza Agarose is quality tested to certify performance
- High gel strength
- Tested for restriction and ligation of recovered DNA fragments
- Standard gelling temperature
Applications
- Ligation
- PCR
- DNA Recovery
- RNA Recovery
Storage and Content
- SeaKem® GTGTM Agarose 125 g
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
Educational Material
Brochures, White Papers etc.
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SourceBook Section VIII - Separation of RNA in Agarose Gels
1. Introduction 2. Preparation of RNA Samples 3. Buffers for RNA Electrophoresis 4. Electrophoresis of RNA 5. Detection of RNA in Agarose Gels with GelStar® or SYBR® Green II Gel Stains 6. Detecting RNA with Ethidium Bromide 7. Northern Blotting 8. FlashGel® System for RNA Analysis 9. References -
SourceBook Section II - Preparation of Agarose Gels
1. Agarose Selection Guide 2. Agarose and Compatible Techniques 3. Agarose Types 4. Agarose Analytical Specifications 5. Suggested Agarose Concentrations & Dye Migration Information 6. Buffers for Electrophoresis 7. Dissolving Agarose 8. Casting Agarose Gels -
SourceBook Section V - In-Gel Reactions
1. Overview 2. Tips for Increasing the Efficiency of In-Gel Reactions 3. Cloning in the Presence of Agarose 4. Restriction Digestion in the Presence of Agarose 5. DNA Amplification in the Presence of Agarose 6. References -
Overview Lonza DNA Markers and Ladders
Select the best DNA Marker for your application -
SourceBook Section IV - Detection and Sizing of DNA in Agarose Gels
1. Guide to Lonza Ladders and Markers 2. Detecting DNA with GelStar® and SYBR® Green Nucleic Acid Gel Stains 3. Detecting DNA with Ethidium Bromide 4. High Sensitivity Detection using the FlashGel® System 5. References -
SourceBook Section III - Loading and Running DNA in Agarose Gels
1. DNA Loading 2. Loading Buffers 3. Optimal Voltage and Electrophoretic Times 4. Fast Running Protocols for High Resolution in MetaPhor® Agarose Gels 5. References -
SourceBook Section VI - Recovery of DNA from Agarose Gels
1. Tips for Increasing DNA Recovery Efficiency from Agarose Gels 2. b-Agarose Recovery of DNA from Agarose Gels 3. Electroelution of DNA from Agarose Gels 4. Phenol/Chloroform Extraction of DNA from Agarose Gels 5. "Modified Freeze/Squeeze" Extraction of DNA from Agarose Gels 6. Ethanol Precipitation of DNA Recovered from Agarose Gels 7. References -
Agarose Selection Guide
Select the best agarose for your application to minimize opportunity for error, optimize results and even reduce costs.
