Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID).
Analytical Specifications
Gelling temperature (1.5%) 36°C ±1.5°C
Melting temperature (1.5%) >90°C
Gel strength (1%) >1,200 g/cm²
Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Request More InfoBenefits
- Wide resolution range (100 bp - 23 kb)
- All Lonza Agarose is quality tested to certify performance
- High gel strength
- Low EEO
- Standard melting temperature
Applications
- Standard DNA/RNA Electrophoreisis
Storage and Content
- SeaKem® LE Agarose 25 g
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
Educational Material
Brochures, White Papers etc.
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SourceBook Section VIII - Separation of RNA in Agarose Gels
1. Introduction 2. Preparation of RNA Samples 3. Buffers for RNA Electrophoresis 4. Electrophoresis of RNA 5. Detection of RNA in Agarose Gels with GelStar® or SYBR® Green II Gel Stains 6. Detecting RNA with Ethidium Bromide 7. Northern Blotting 8. FlashGel® System for RNA Analysis 9. References -
SourceBook Section II - Preparation of Agarose Gels
1. Agarose Selection Guide 2. Agarose and Compatible Techniques 3. Agarose Types 4. Agarose Analytical Specifications 5. Suggested Agarose Concentrations & Dye Migration Information 6. Buffers for Electrophoresis 7. Dissolving Agarose 8. Casting Agarose Gels -
SourceBook Section IX - Special Applications in Agarose Gels
1. Amplification of Plasmid cDNA Libraries with SeaPrep® Agarose 2. Preparing Agarose for use in Cell Culture Applications 3. References -
Overview Lonza DNA Markers and Ladders
Select the best DNA Marker for your application -
SourceBook Section IV - Detection and Sizing of DNA in Agarose Gels
1. Guide to Lonza Ladders and Markers 2. Detecting DNA with GelStar® and SYBR® Green Nucleic Acid Gel Stains 3. Detecting DNA with Ethidium Bromide 4. High Sensitivity Detection using the FlashGel® System 5. References -
SourceBook Section III - Loading and Running DNA in Agarose Gels
1. DNA Loading 2. Loading Buffers 3. Optimal Voltage and Electrophoretic Times 4. Fast Running Protocols for High Resolution in MetaPhor® Agarose Gels 5. References -
Agarose Selection Guide
Select the best agarose for your application to minimize opportunity for error, optimize results and even reduce costs.
