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Troubleshooting Guide - PYROGENT® Gel Clot LAL AssayMost problems encountered in the use of the PYROGENT® Gel Clot Limulus Amebocyte Lysate (LAL) Assay fall into two basic categories - no firm gels are observed in reaction tubes where firm gels are expected or firm gels form in reaction tubes, which are expected to yield negative results. This Tech Tip takes a deeper look into each of these categories and provides troubleshooting guidance.
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Instructions - PYROGENT™ and PYROGENT™ Plus (Chinese)Detailed instructions for performing the PYROGENT™ and PYROGENT™ Plus Gel Clot LAL Assay
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Instructions - PYROGENT® and PYROGENT® Plus (English)Detailed instructions for performing the PYROGENT® and PYROGENT® Plus Gel Clot LAL Assay
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (German)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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How To Perform The PYROGENT™ Gel Clot LAL AssayThe gel clot LAL assay is a qualitative test that provides simple positive-negative results. This video demonstrates how to perform the assay from start to finish.Watch Video -
Troubleshooting Guide - Testing Oil Solutions with PYROGENT™ Gel Clot LAL AssayEndotoxins, due to their lipid character, tend to remain associated with oils and do not readily enter the aqueous phase of water-oil mixtures. This Tech Tip describes the use of a dispersing agent, PYROSPERSE™, to allow for dissociation of the endotoxin from the oils and for subsequent detection of endotoxin in the aqueous phase by the PYROGENT™ Gel Clot LAL Assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (English)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (French)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Japanese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Portuguese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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