The SE Cell Line 4D-Nucleofector^® X Kit L is one of our three 4D-Nucleofector^® Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette^® Vessel format when working with the 4D-Nucleofector^® X Unit.

This kit has been determined to be optimal for transfecting following cell types:

3T3-L1, diff., 3T3-L1, undiff.
A-10, A7r5,
BHK-21, BJ
C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
DU145
GH3
HCT116, HeLa, HeLa S3
IMR-90
Jurkat (DSMZ), Jurkat E6.1
KG-1a, L6
MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
NCTC clone 929
Panc-1
T/G-HA VSMC, T-47D
U2OS, U-87 MG
WI-38
T84

The respective cell type-specific Optimized Protocols are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.

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Benefits

High transfection efficiencies in difficult-to-transfect cell lines
Cells maintain functionality post transfection
Conditions are transferable between 4D-Nucleofector^® System (including 4D-Nucleofector^® LV Unit), 96-well Shuttle^® System and 384-well Nucleofector^® System

Applications

Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cells
Low throughput applications

Storage and Content

2.25 mL SE Cell Line Nucleofector^® Solution
0.5 mL Supplement 1
50 µg pmaxGFP^TM Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL Nucleocuvette^® Vessel
24 x Single use pipettes

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SDS, CoA, and Instructions

Safety Data Sheets (SDS)

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Language:

German

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Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

4D-Nucleofector® Protocol for MRC-5
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MRC-5

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4D-Nucleofector® Protocol for IMR90
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for IMR90

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4D-Nucleofector® Protocol for Jurkatclone E6.1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Jurkatclone E6.1

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4D-Nucleofector® Protocol for T-47D
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for T-47D

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4D-Nucleofector® Protocol for Calu-3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Calu-3

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4D-Nucleofector® Protocol for A7r5
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A7r5

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4D-Nucleofector® Protocol for MDA-MB-468
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MDA-MB-468

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4D-Nucleofector® Protocol for HeLa cells
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HeLa cells

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4D-Nucleofector® Protocol for HCT116
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HCT116

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4D-Nucleofector® Protocol for BJ
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for BJ

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4D-Nucleofector® Protocol for A10
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A10

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4D-Nucleofector® Protocol for DU1345
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for DU1345

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4D-Nucleofector® Protocol for C2C12
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for C2C12

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4D-Nucleofector® Protocol for TG HA-VSMC
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for TG HA-VSMC

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4D-Nucleofector® Protocol for MEG-01
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MEG-01

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4D-Nucleofector® Protocol for WI-38
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for WI-38

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4D-Nucleofector® Protocol for PANC-1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for PANC-1

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4D-Nucleofector® Protocol for GH3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for GH3

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4D-Nucleofector® Protocol for Caco-2
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Caco-2

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4D-Nucleofector® Protocol for KG-1a
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for KG-1a

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4D-Nucleofector® Protocol for U-87 MG
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for U-87 MG

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4D-Nucleofector® Protocol for HeLa S3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HeLa S3

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4D-Nucleofector® Protocol for MDCK II
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MDCK II

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4D-Nucleofector® Protocol for 3T3-L1adipocytes
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for 3T3-L1adipocytes

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4D-Nucleofector® Protocol for Capan-1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Capan-1

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4D-Nucleofector® Protocol for U20S
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for U20S

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4D-Nucleofector® Protocol for MDA-MB-231
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MDA-MB-231

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4D-Nucleofector® Protocol for BHK-21
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for BHK-21

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4D-Nucleofector® Protocol for NCTC clone 929
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for NCTC clone 929

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4D-Nucleofector® Protocol for MDCK
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MDCK

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4D-Nucleofector® Protocol for 3T3-L1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for 3T3-L1

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4D-Nucleofector® Protocol for NK-92
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for NK-92

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4D-Nucleofector® Protocol for MG-63
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MG-63

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4D-Nucleofector® Protocol for L6
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for L6

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4D-Nucleofector® Protocol for COS-7
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for COS-7

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