Human CD3+ T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 25 million cells, cryopreserved
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (100 µL format).
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For adherent electroporation of primary cells, e.g. neuronal or endothelial cells, when working with the 4D-Nucleofector® Y Unit.
Cryopreserved ampule of Human Mammary Epithelial Cells (HMEC) containing ≥500,000 cells
Culture system containing REBM® Basal Medium (CC-3191) and REGM® SingleQuots® supplements (CC-4127) required for growth of Renal Epithelial Cells.
For medium-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 4D-Nucleofector® 96-well Unit
For high-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 384-well HT Nucleofector® System.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
CD34+ Cells for Cell and Gene Therapy Process DevelopmentIn this interview Dr. Manon Destalminil, Bioprocessing Project Leader at CellProthera, discusses the advantages of using research-grade CD34+ cells for cell and gene therapy process development. Please register to read.
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