For efficient transfection of specific primary cells, e.g. HUVEC, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-Nucleofector® X Unit (100 µL format).
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit.
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (20 µL format).
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-Nucleofector® X Unit (20 µL format).
For testing the functionality of your 4D-Nucleofector® X Unit for use with 100 µL Nucleocuvette® Vessels
For testing the functionality of your 4D-Nucleofector® X Unit for use with 20 µL Nucleocuvette® Strips
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit