P1 Primary Cell 384-well Nucleofector™ Kit
P1 Primary Cell 384-well Nucleofector™ Kit
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Product Overview
The P1 Primary Cell 384-well NucleofectorTM Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT NucleofectorTM System.
This kit has been determined to be optimal for transfecting following cell types:
- ADSC undiff. (human)
- MSC (human)
- Preadipocytes (human)
- PrEC (human)
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.
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Benefits
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
- Seamless integration into automated liquid handling environments
- Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
Applications
- Transfection of low cell numbers down to 2 x 104 primary cells
- High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Storage and Content
22.5 mL P1 Primary Cell NucleofectorTM Solution
5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 384-well NucleocuvetteTM Plate (20 μl)
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Transfection Protocol for Human MSC - HT Nucleofector™ Device
Instructions of use for transfection of human mesenchymal stem cells with the P1 Primary Cells HT-Nucleofector™ Kit -
Transfection Protocol for Human PrEC - HT Nucleofector™ Device
Instructions of use for transfection of human prostate epithelial cells (Lonza) with the P1 Primary Cell HT-Nucleofector™ Kit -
Basic Transfection Protocol for Epithelial Cells - HT Nucleofector™ Device
Instructions of use for transfection of primary mammalian epithelial cells with the P1 or P3 Primary Cell HT-Nucleofector™ Kit -
Basic Transfection Protocol for SMC - HT Nucleofector™ Device
Instructions of use for transfection of primary mammalian smooth muscle cells with the P1 Primary Cell HT Nucleofector™ Kit
