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CRISPR in the Classroom

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Article overview

Reprogramming of human erythroblasts into iPSC for Sickle Cell Disease research

The Nucleofection® Technology used for generation of three SCD-iPS and three control iPS cell lines.

Using longer single guides to increase editing efficiency

The authors describe a novel approach that leads to higher editing efficiency in CRISPR experiments

CAR-T therapy overview

CAR-T therapy is a new cutting edge treatment involving modification of human T cells, but also has some challenges to overcome. Learn more about it in a brief overview of this therapy.

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References

Glacer A, McColl B, Vadolas J. GFP to BFP Conversion: A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing Molecular Therapy - Nucleic Acids (2016) 5, e334

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