Product Overview
The Primary Cell Optimization Nucleofector® Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit.
With the unique capability of the 4D-Nucleofector® 96-well Unit to address individual wells of a 96-well Nucleocuvette® Plate with different programs, primary cell optimizations are easily performed within one experiment.
Complete Optimization: Using one 96-well Nucleocuvette® Plate and our pmaxGFPTM Vector as positive control, our five Primary Cell Nucleofector® Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well Nucleocuvette® Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs. Basic Protocols are available for following cell groups:
- Endothelial cells (mammalian)
- Epithelial cells (mammalian)
- Fibroblasts (mammalian)
- Glial cells (mammalian)
- Neurons (mammalian)
- Smooth muscle cells (mammalian)
- Stem cells (human)
Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
Request More InfoBenefits
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined on the 4D-Nucleofector® 96-well Unit are transferable to the 4D-Nucleofector® System or the 384-well HT Nucleofector® System
Applications
- Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit
Storage and Content
0.675 mL Nucleofector® Solution P1
0.675 mL Nucleofector® Solution P2
0.675 mL Nucleofector® Solution P3
0.675 mL Nucleofector® Solution P4
0.675 mL Nucleofector® Solution P5
4 x 0.15 mL Supplement 1
1 x 0.15 mL Supplement 3
2 x 50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 96-well Nucleocuvette® Plates
