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Inspection Equipment Nucleofector® II/2b System
Catalog #: AXP-1001
For testing the functionality of your Nucleofector® 2b System
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Cell Line Optimization 384-well Nucleofector® Kit
Catalog #: V5SC-9001
For high-throughput optimization of transfection conditions for cell lines lacking an Optimized Protocol for the 384-well HT Nucleofector® System
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Inspection Equipment 96-well Shuttle® Device
Catalog #: AXP-1002
For testing the functionality of your 96-well Shuttle® Add-On
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Pipette Tips, 100–1250 µL
Catalog #: BE25-414
LAL Reagent GradeTMPipette Tips, 100–1250 µL, <0.005 EU/mL, 10 × 96 tips. Ideal for use in the endotoxin test.
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Pipette Tips, 2–200 µL
Catalog #: BE25-413
LAL Reagent GradeTMPipette Tips, 2–200 µL, <0.005 EU/mL, 10 × 96 tips. Ideal for use in the endotoxin test.
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Inspection Equipment 4D-Nucleofector® 16-well Strip
Catalog #: AXP-1004
For testing the functionality of your 4D-Nucleofector® X Unit for use with 20 µL Nucleocuvette® Strips
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Instructions - QCL-1000™ Assay (English)Detailed instructions for performing the QCL-1000™ Endpoint Chromogenic LAL Assay
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PYROSTAR™ ES-F Series BrochureThe PYROSTAR™ ES-F series of reagents are endotoxin-specific and unreactive to β-D-Glucan
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QCL-1000™ Endpoint Chromogenic LAL Assay - Technical TipsThe Importance of Glass Tubes /The Problem with PlasticIncubation Method96-well Plates1 EU/ml Standard PreparationVortexing and Mixing Potential Endotoxin Contaminants
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Instructions – Water for Cell CultureData sheet WFI
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Troubleshooting Guide - Testing Oil Solutions with PYROGENT® Gel Clot LAL AssayEndotoxins, due to their lipid character, tend to remain associated with oils and do not readily enter the aqueous phase of water-oil mixtures. This Tech Tip describes the use of a dispersing agent, PYROSPERSE®, to allow for dissociation of the endotoxin from the oils and for subsequent detection of endotoxin in the aqueous phase by the PYROGENT® Gel Clot LAL Assay.
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Troubleshooting Guide - Endpoint Chromogenic LAL Assay Procedure (English)This is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate.
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Instructions - MycoZap™ Mycoplasma Elimination ReagentMycoZap™ Mycoplasma Elimination Reagent Instructions
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Endpoint Chromogenic Limulus Amebocyte Lysate (LAL) Assay Procedure Quick GuideThis is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate. (English)
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Overcoming Assay Inhibition or Enhancement Technical TipsOne of the most time-consuming aspects of endotoxin testing using Limulus Amebocyte Lysate (LAL) is pretreating samples to overcome assay inhibition and enhancement. All assays, independent of methodology, are standardized using endotoxin in water. Lonza offers three solutions for overcoming LAL testing inhibitions
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White-Paper LAL-vs-rFCLonza discusses the transistion From LAL to rFC Assays
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