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Human Mammary Epithelial Cell Nucleofector™ Kit

Human Mammary Epithelial Cell Nucleofector™ Kit

Catalog #: VPK-1002
For efficient transfection of primary human mammary epithelial cells in the NucleofectorTM I/II/2b Device
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Product Overview

The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This NucleofectorTM Kit is the optimal kit for efficient transfection of primary human mammary epithelial cells.

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.

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Benefits

  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection

Applications

  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells 
  • Low-throughput transfection in single cuvette format

Storage and Content

2.25 mL NucleofectorTM Solution for Mammary Epithelial Cells
0.5 mL Supplement 3
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Transfection Protocol for HMEC - Nucleofector™ I/II/2b Device Series

    Instructions of use for transfection of human mammary epithelial cells (Lonza) with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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