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4D-Nucleofector^® Y Unit

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4D-Nucleofector^® Y Unit

Catalog #: AAF-1003Y

Enables the direct Nucleofection^® of cells in adherence in 24-well culture plates

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Product Overview

Adherent Nucleofection^® Using the Y Unit

Electroporation-based methods have so far required cells to be in suspension for transfection. The Nucleofector^® Technology entered a new era and allows direct Nucleofection^® Reactions of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofector^® Technology in their physiological state. The Y Unit of the 4D-Nucleofector^® System works with disposable conductive polymer Dipping Electrode Arrays that can be inserted into standard 24-well culture plates for Nucleofection^® Procedure.

The 4D-Nucleofector^® Y Unit is one of the four functional modules of the 4D-Nucleofector^® System.

There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection^® Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Technical Specifications

Dimensions: (w x h x d): 24.5×10.5×28 cm / 9.7×4.1×11.0

Weight: 5.0 kg (11.0 lb)

Power: 100 - 240 VAC

Frequency 50 - 60 Hz

Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)

Fuse: T 2.5A, L250V

Operating temperature: +15°C up to +40°C (59°F - 104°F)

Environment non condensing

Voltage limitation: 1500 V

Current limitation: 100 A

Compatibility

This functional module can be operated with all versions of the Core Unit (part code AAF-1001B, AAF-1002B, AAF-1003B)

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Benefits

Pre- and post Nucleofection^® culture in 24-well culture plates

Nucleofection^® of cells at any time point during this culture period, i.e. at a later developmental stage
Transfection efficiencies up to 70% combined with high viabilities
Compatible with Clonetics^® Primary Animal Neurons

Storage and Content

1 Unit

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SDS, CoA, and Instructions

Safety Data Sheets (SDS)

SDS not applicable

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA.

View Document
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results.

View Document
4D-Nucleofector® Quick Guide
Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment

View Document
4D-Nucleofector® Hardware Manual
Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1

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Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections.

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Nucleofector® Technology Brochure
Stretching Transfection Dimensions

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Educational Material

Brochures, White Papers etc.

Videos

Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA.

View Document
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results.

View Document
4D-Nucleofector® Quick Guide
Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment

View Document
4D-Nucleofector® Hardware Manual
Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1

View Document
Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections.

View Document
Adherent Nucleofection of Cryopreserved Dissociated Rat Brain Neuronal Cells
Results for transfecting cultured dissociated rat brain neurons (hippocampal or cortical) using the 4D-Nucleofector Y Unit. It compares results for freshly isolated neurons with Lonza cryopreserved neurons.

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Citation List
A list of selected and reviewed publications for the Nucleofector Technology with focus on 4D-Nucleofector System.

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4D-Nucleofector® Platform - The Story Of
See how breakthrough Nucleofector® Technology innovations in transfection and electroporation culminate in the next generation 4D-Nucleofector® System.

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How it Works - 4D-Nucleofector® System (Video Tutorial)
Workflow overview for using the 4D-Nucleofector™ System

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How it Works - 4D-Nucleofector® Y Unit (Video Tutorial)
Workflow overview for using the 4D-Nucleofector™ Y Unit

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