Basic Nucleofector® Kit for Primary Mammalian Fibroblasts
Basic Nucleofector® Kit for Primary Mammalian Fibroblasts
For transfection of primary mammalian fibroblasts lacking a cell type specific Optimized Protocol for the Nucleofector® I/II/2b Device
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Product Overview
In addition to various cell type-specific kits for primary fibroblasts, we also provide a cell group-specific Basic Nucleofector® Kit. This kit is suited to determine the optimal transfection conditions for any mammalian fibroblast type that lacks a cell type specific Optimized Protocol for the Nucleofector® 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector® Programs is tested in combination with the Basic Nucleofector® Solution for fibroblasts.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Request More InfoBenefits
- Optimization can be performed within 1 experiment
- Detailed protocols guiding through the optimization procedure
- Optional result fine tuning with help of our Scientific Support Team
- Transfection efficiencies up to 90%
- Viabilities up to 98%
Applications
- Kit suited for fibroblasts from different mammalian species and various organs
- Already tested for macaque dermal fibroblasts, bovine fibroblasts, human colon myofibroblasts, mouse lung fibroblasts, etc.
Storage and Content
2.25 mL Nucleofector® Solution for Primary Mammalian Fibroblasts
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
