Product Overview
NuSieve® GTG® Agarose is a low melting temperature (65º C) agarose that finely resolves DNA fragments, PCR and RT-PCR products ranging from 10 to 1,000 bp. NuSieve® GTG® Agarose forms easy-to-handle gels and provides consistent DNA mobility from lot-to-lot and is tested for the presence of proteases, ligases and nucleases. Cloning procedures can be performed directly in remelted NuSieve® GTG® Agarose, eliminating costly and time-consuming DNA extraction steps. This agarose is also compatible with reactions carried out in the presence of the remelted agarose gel. NuSieve® GTG® Agarose is tested and certified for reliable ligation and transformation of DNA directly in a remelted agarose gel.
Analytical Specifications
Gelling temperature (3%) ≤35°C
Melting temperature (3%) ≤65°C
Gel strength (3%) ≥500 g/cm²
Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Request More InfoBenefits
- All Lonza Agarose is quality tested to certify performance
- Fine resolution of small DNA fragments
- Performance certified for digestion and ligation
- Low EEO
- Low melting temperature
- DNase/RNase Free
Applications
- Ligation
- PCR
- DNA Recovery
- RNA Recovery
- Small Fragment
Storage and Content
- NuSieve® GTG® Agarose 125 g
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
Educational Material
Brochures, White Papers etc.
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SourceBook Section I - Frequently Asked Questions
1. Nucleic Acid FAQs 2. Protein Analysis FAQs -
SourceBook Section VIII - Separation of RNA in Agarose Gels
1. Introduction 2. Preparation of RNA Samples 3. Buffers for RNA Electrophoresis 4. Electrophoresis of RNA 5. Detection of RNA in Agarose Gels with GelStar® or SYBR® Green II Gel Stains 6. Detecting RNA with Ethidium Bromide 7. Northern Blotting 8. FlashGel® System for RNA Analysis 9. References -
SourceBook Section II - Preparation of Agarose Gels
1. Agarose Selection Guide 2. Agarose and Compatible Techniques 3. Agarose Types 4. Agarose Analytical Specifications 5. Suggested Agarose Concentrations & Dye Migration Information 6. Buffers for Electrophoresis 7. Dissolving Agarose 8. Casting Agarose Gels -
SourceBook Section V - In-Gel Reactions
1. Overview 2. Tips for Increasing the Efficiency of In-Gel Reactions 3. Cloning in the Presence of Agarose 4. Restriction Digestion in the Presence of Agarose 5. DNA Amplification in the Presence of Agarose 6. References -
Overview Lonza DNA Markers and Ladders
Select the best DNA Marker for your application -
SourceBook Section IV - Detection and Sizing of DNA in Agarose Gels
1. Guide to Lonza Ladders and Markers 2. Detecting DNA with GelStar® and SYBR® Green Nucleic Acid Gel Stains 3. Detecting DNA with Ethidium Bromide 4. High Sensitivity Detection using the FlashGel® System 5. References -
SourceBook Section III - Loading and Running DNA in Agarose Gels
1. DNA Loading 2. Loading Buffers 3. Optimal Voltage and Electrophoretic Times 4. Fast Running Protocols for High Resolution in MetaPhor® Agarose Gels 5. References -
SourceBook Section VI - Recovery of DNA from Agarose Gels
1. Tips for Increasing DNA Recovery Efficiency from Agarose Gels 2. b-Agarose Recovery of DNA from Agarose Gels 3. Electroelution of DNA from Agarose Gels 4. Phenol/Chloroform Extraction of DNA from Agarose Gels 5. "Modified Freeze/Squeeze" Extraction of DNA from Agarose Gels 6. Ethanol Precipitation of DNA Recovered from Agarose Gels 7. References -
Agarose Selection Guide
Select the best agarose for your application to minimize opportunity for error, optimize results and even reduce costs.
