Kinetic-QCL™ Kinetic Chromogenic LAL Assay, 192 Test Kit
Kinetic-QCL™ Kinetic Chromogenic LAL Assay, 192 Test Kit
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Product Overview
The Kinetic-QCLTM Kinetic Chromogenic LAL Assay is a quantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent in a 96-well plate and placed in an incubating plate reader that measures absorbance at 405 nm. The reaction is automatically monitored over time for the appearance of a yellow color.
In the presence of endotoxin, the lysate will begin to cleave the chromogenic substrate, causing the solution to become yellow. The time required for the change is inversely proportional to the amount of endotoxin present. The concentration of unknown samples can then be calculated from a standard curve.
It is the chromogenic nature of the Kinetic-QCLTM Kinetic Chromogenic LAL Assay that makes it the most appropriate choice for testing small volume parenterals, vaccines, antibiotics and biologicals. The Kinetic-QCLTM Assay is less impacted by inhibitory products which may interfere with the clotting mechanism in turbidimetric and gel clot assays. With a sensitivity range of 0.005 EU/ml - 50.0 EU/ml, the Kinetic-QCLTM Kinetic Chromogenic LAL Assay is the most sensitive of the LAL assays.
Bulk configurations are available as made-to-order products.
Benefits
- Most sensitive LAL-based method
- Less sensitive to product inhibition
Applications
- Ideal for biological products such as vaccines and antibiotics
Storage and Content
- 8 x 24 tests/vial LAL lysate
- 2 vials endotoxin
- 3 x 30 mL/vial LAL Reagent Water
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Instructions - Limulus Amebocyte Lysate (LAL) Kinetic-QCL® (English)
Detailed instructions for performing the Kinetic-QCL® Kinetic Chromogenic LAL Assay in English -
Troubleshooting Guide - Kinetic LAL Assay Procedure (English)
A step-by-step guide depicting how to perform a traditional kinetic LAL assay.
Educational Material
Brochures, White Papers etc.
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Troubleshooting Guide - Testing Oil Solutions with the PYROGENT™-5000 and Kinetic-QCL™ Assays
Oil-based parenterals have always been a challenge in endotoxin detection assays. This Tech Tip describes a liquid-liquid extraction procedure that can be used to remove the endotoxin from the oil into an aqueous phase, which can then be tested with the PYROGENT™-5000 or Kinetic-QCL™ Assays. -
Instructions - Limulus Amebocyte Lysate (LAL) Kinetic-QCL® (English)
Detailed instructions for performing the Kinetic-QCL® Kinetic Chromogenic LAL Assay in English -
Troubleshooting Guide - Kinetic LAL Assay Procedure (English)
A step-by-step guide depicting how to perform a traditional kinetic LAL assay. -
Technical Tip - Serum, Plasma Testing with PYROGENT® 5000 and Kinetic-QCL® Assays
The article focuses on the recommended procedure that can be used to prepare serum or plasma for LAL-based endotoxin testing.