The PYROGENT^TM Gel Clot LAL Assay is a qualitative endotoxin detection test that gives a positive/negative result. If endotoxin is present, a gel clot will form and stay intact in a test tube when inverted 180°.

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Benefits

Sensitivity of 0.25 EU/ml Easy-to-read qualitative results No need to purchase CSE separately

Applications

Simple LAL test not requiring sophisticated instrumentation and software

Storage and Content

80 x 50 tests/vial lysate

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Educational Material

Brochures, White Papers etc.

Technical Tips - PYROGENT® Gel Clot
QC testing professionals and researchers sometimes encounter issues when performing the gel clot LAL test. This Tech Tip will explore ways to help overcome these challenges including the use of appropriate accessory items, correct incubation method and proper technique for flipping the tubes.

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Troubleshooting Guide - PYROGENT® Gel Clot LAL Assay
Most problems encountered in the use of the PYROGENT® Gel Clot Limulus Amebocyte Lysate (LAL) Assay fall into two basic categories - no firm gels are observed in reaction tubes where firm gels are expected or firm gels form in reaction tubes, which are expected to yield negative results. This Tech Tip takes a deeper look into each of these categories and provides troubleshooting guidance.

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Instructions - PYROGENT® and PYROGENT® Plus (English)
Detailed instructions for performing the PYROGENT® and PYROGENT® Plus Gel Clot LAL Assay

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Troubleshooting Guide - Testing Oil Solutions with PYROGENT™ Gel Clot LAL Assay
Endotoxins, due to their lipid character, tend to remain associated with oils and do not readily enter the aqueous phase of water-oil mixtures. This Tech Tip describes the use of a dispersing agent, PYROSPERSE™, to allow for dissociation of the endotoxin from the oils and for subsequent detection of endotoxin in the aqueous phase by the PYROGENT™ Gel Clot LAL Assay.

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Troubleshooting Guide - Gel Clot LAL Assay Procedure (English)
A step-by-step guide depicting how to perform a traditional gel clot LAL assay.

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