PASMC - Human Pulmonary Artery Smooth Muscle Cells
PASMC - Human Pulmonary Artery Smooth Muscle Cells
Cryopreserved ampule of Pulmonary Artery Smooth Muscle Cells (PASMC) containing ≥ 500,000 cells
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Product Overview
Human Pulmonary Artery Smooth Muscle Cells (PASMC) are shipped in 3rd passage.
Human Pulmonary Artery Smooth Muscle Cells are guaranteed for 15 population doublings and to stain positive for alpha smooth muscle actin and negative for von Willebrand Factor VIII after differentiation.
All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.
Request More InfoApplications
- Stroke
- Diabetes
- Vascular research
- Artherosclerosis
- Hypertension
Storage and Content
1 x Cryopreserved ampule PASMC containing ≥500,000 cells/vial
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Instructions - Human Smooth Muscle Cell Systems
Instructions for culturing Clonetics™ Human Smooth Muscle Cells -
TechSheet - Pulmonary Artery Smooth Muscle Cell Systems
Technical Information Sheet for Clonetics™ Pulmonary Artery Smooth Muscle Cell Systems
Educational Material
Brochures, White Papers etc.
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Airway Cell and Media Reference Guide -
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Primary Cells for Airway Research – Infographic
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Supporting In Vitro ADMETox with an Advanced Portfolio of Primary Cells and Media
Reference to Help Get Started on Primary Cell Culture for ADMETox Applications
PASMC - Human Pulmonary Artery Smooth Muscle Cells
Human pulmonary artery smooth muscle cells(CC-2581) were stained with 1:1500 dilution of Anti Alpha-Smooth Muscle Actin dilution of (CBL171, Sigma). Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at a 1:200 dilution. Nuclei were stained with Hoechst . In multi-well culture, PAMCSs appeared typically epitheloid (polygonal) forming monolayers of very low-level alpha-actin expressing cells. Rarely, a few intensely alpha-actin positive cells (arrows) were evident. These displayed distinct fusiform-elongated morphology.
PASMC - Human Pulmonary Artery Smooth Muscle Cells
Cells are isolated from human pulmonary artery and expanded prior to freezing (CC-2581). When plated & expanded, the cells express little to no alpha-actin unless first stimulated to differentiate.Cells were stained with Anti Alpha-Smooth Muscle Actin Chemicon (Millipore) CBL171 at 1:1500 dilution. Immunofluorescence analysis was conducted using Secondary donkey polyclonal antibody Alexa Fluor Do anti Mo 594 Invitrogen (Thermo Fisher) A21203 at 1:200 dilution. Nuclei were stained with Hoechst.
