The PyroCell® Monocyte Activation Test – Human Serum Kit combines 3 vials of cryopreserved, pooled PBMC (human, pMAT cells), and 3 vials of cryopreserved Human Serum Supplement, for 288 tests (3x 96-well microtiter plates).
The PeliKine Human IL-6 ELISA Rapid Kit combines two reagent kit boxes: the PeliKine Human IL-6 ELISA Rapid Set A (M2018) and the PeliKine Human IL-6 ELISA Rapid Set B (M2019). The PeliKine Human IL-6 ELISA Rapid Kit contains all necessary reagents and buffers to perform 288 test (3 ELISA plates). It is used in combination with the PyroCell® MAT Kit (# 249735) or PyroCell® MAT Human Serum Kit (# 279770) to conduct the Monocyte Activation Test (MAT).
Human CD3+ T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 25 million cells, cryopreserved
X-VIVO® 15, Serum-free hematopoietic cell medium, with L-Glutamine, gentamicin and phenol red, xenofree, 1 L
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Cryopreservation of Peripheral Blood Mononuclear CellsDoes Not Alter Immune Cell Phenotype
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Predicting Antibody Dependent Cell-Mediated Cytotoxicity (ADCC)Genotypic and Phenotypic Screening of Human Peripheral Blood Mononuclear Cells
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Two Distinct Activation Methods Yield Clinical-Scale Expansion of Peripheral Blood Derived γδ T CellsIn this study, we demonstrate that billions of γδ T cells can be generated from peripheral blood mononuclear cells (PBMCs) with two distinct activation methods, using TheraPEAK® T-VIVO® Medium.
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Normal Peripheral Blood CD4+ T LymphocytesLonza Application Notes
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B-ALI Immune-Airway Co-Culture Model: Normal Human Bronchial Epithelial cells and Peripheral Blood Mononuclear Cells - Instructions for useProtocol to culture B-ALI NHBE cells with PBMCs
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Validation of Th17 Cell Differentiation from Peripheral BloodLonza White Paper
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siRNA Transfection Protocol for Blood LymphocytesInstructions of use for transfection of human peripheral blood lympohocytes with siRNA using the Nucleofector™ Technology
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Transfection Protocol for Human T Cells (Stim.) - HT Nucleofector™ DeviceInstructions of use for transfection of stimulated peripheral blood human T cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Tips For Optimizing Your CD8+ T Cell and Dendritic Cell Co-culturesA co-culture between matured dendritic cells (DCs) and CD8+ T cells isolated from Lonza cryopreserved human peripheral blood mononuclear cells (PBMCs; part nos. CC-2702 – CC-2705) and we present three major takeaways from this process that are critical for successful co-culture and end-point analysis for these two cell types
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Transfection Protocol for Human T Cells (Stim.) - 96-well Shuttle™ DeviceInstructions of use for transfection of stimulated peripheral blood human T cells with the P3 Primary Cell 96-well Nucleofector™ Kit