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SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit™

SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit™

Catalog #: CC-3118

Culture system containing SABMTM Basal Medium (CC-3119) and SAGMTM SingleQuotsTM supplements (CC-4124) required for growth of Small Airway Epithelial cells

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 419.00 CHF
SA

Product Overview

SAGMTM BulletKitTM is small airway epithelial cell growth medium that is serum-free and has been optimized to grow human small airway epithelial cells (SAEC) from normal and diseased tissue.                                      

Published research shows SAGMTMTM BulletKitTM Small Airway Epithelial Cell Growth Medium is also suitable to support co-culture experiments with primary mammary epithelial cells (HMEC) as well as established breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the relevant published data in the 'Data' tab.

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Storage and Content

1 x SABMTM Basal Medium (CC-3119), 500 mL

1 x SAGMTM SingleQuotsTM Supplement Pack (CC-4124) containing:

1 x Orange Cap Vial with BPE, 2.0 mL
1 x Lilac Cap Vial with Insulin, 0.5 mL
1 x Natural Cap Vial with Hydrocortisone, 0.5 mL
1 x Red Cap Vial with GA-1000, 0.5 mL
1 x Amber Vial with Retinoic Acid, 0.5 mL
1 x Bottle with Fatty Acid Free BSA, 5 mL
1 x Natural Cap Vial with Transferrin, 0.5 mL
1 x Amber Vial with Triiodothyronine, 0.5 mL
1 x Amber Vial with Epinephrine, 0.5 mL
1 x Green Cap Vial with hEGF, 0.5 mL

Download our Airway Cell Culture Media Concentration Guide

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Instructions - Airway Epithelial Cell Systems

    Instructions for Proper Use and Culture of Airway Epithelial Cells
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
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Educational Material

Brochures, White Papers etc.

  • Lonza's Bulletkit™ for Primary Cell Growth

    Primary Cells are Different from Cell Lines. Why use the Same Classical Media? Learn how Lonza primary cells and media can support your cell research
  • Primary Human Airway Cells and Media

    Airway Cell and Media Reference Guide
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • An Air-liquid Interface Culture System for Small Airway Epithelial Cells

  • Primary Cells for Airway Research – Infographic

    Infographic showing how Lonza primary cells can aide in your airway research
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Data

Co-culture Experiments with Lonza's Small Airway Epithelial Cells and SAGM™ BulletKit™ Media

Data Courtesy of Furukawa, et al. Lonza's SAEC cells were co-cultured with primary normal mammary epithelial cells, breast cancer cell lines MCF-7 and MDA-MB-231 in Lonza's SAGM™ BulletKit™ Growth Media. Breast cancer cell lines showed differences in morphology and proliferation rates in the presence of small airway epithelial cells. The image above shows phase contrast imaging with a fluorescent (red) overlay of MCF-7 cell line. MCF-7 cell lines survived as tight clusters in SAGM™ Media. When co-cultured with Lonza's primary SAECs, MCF-7 cells demonstrated enhanced growth all the while staying clustered.

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