For efficient transfection of cell lines, e.g. 3T3-L1 ad, DU145, EL4, IMR-32 or MDCK, in the Nucleofector® I/II/2b Device
TGF Beta-3 required for chondrogenic differentiation, 2 µg. For use with the hMSC Chondrogenic Differentiation BulletKit®, PT-3003 (sold separately)
Cryopreserved ampule of Human Subcutaneous Preadipocytes containing ≥1 million cells
Cryopreserved Human Adult Epidermal Keratinocytes (NHEK-Ad) from a single donor, ≥ 500,000 cells
OsteoImageTM Mineralization Assay is a rapid, fluorescent in vitro assay for assessing bone cell mineralization, 500 tests.
For efficient transfection of primary human macrophages in the Nucleofector® I/II/2b System
For determination of the optimal transfection conditions for any difficult-to-transfect cell line that lacks an Optimized Protocol for the Nucleofector® I/II/2b Device
For efficient transfection of human stem cells in the Nucleofector® I/II/2b Device
-
Troubleshooting Guide - PYROGENT® Gel Clot LAL AssayMost problems encountered in the use of the PYROGENT® Gel Clot Limulus Amebocyte Lysate (LAL) Assay fall into two basic categories - no firm gels are observed in reaction tubes where firm gels are expected or firm gels form in reaction tubes, which are expected to yield negative results. This Tech Tip takes a deeper look into each of these categories and provides troubleshooting guidance.
-
Troubleshooting Guide - Gel Clot LAL Assay Procedure (English)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
-
Troubleshooting Guide - Gel Clot LAL Assay Procedure (French)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
-
Troubleshooting Guide - Gel Clot LAL Assay Procedure (Japanese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
-
Troubleshooting Guide - Gel Clot LAL Assay Procedure (Portuguese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
-
Troubleshooting Guide - Gel Clot LAL Assay Procedure (Spanish)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
-
Troubleshooting Guide - Gel Clot LAL Assay Procedure (Italian)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
-
Troubleshooting Guide - Gel Clot LAL Assay Procedure (Chinese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
-
How To Perform The PYROGENT™ Gel Clot LAL AssayThe gel clot LAL assay is a qualitative test that provides simple positive-negative results. This video demonstrates how to perform the assay from start to finish. -
Instructions - PYROGENT™ and PYROGENT™ Plus (Chinese)Detailed instructions for performing the PYROGENT™ and PYROGENT™ Plus Gel Clot LAL Assay