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Hematopoietic Stem Cell Therapy

Hematopoietic stem cells (HSCs) are multipotent precursors that can renew and have the ability to regenerate mature blood cells through hematopoiesis. Hematopoietic stem cells (HSCs) play a crucial role in hematopoiesis. HSCs, which are generally found in the bone marrow, are constantly self-renewing and differentiating into all the different types of blood cells. During this process, HSCs leave the bone marrow and enter the peripheral blood and tissues. Here, they progress through two different progenitor stages (lymphoid and myeloid progenitors) before becoming mature blood cell types:

Myeloid progenitor cells can give rise to the myeloid lineage including platelets, eosinophils, basophils, neutrophils, monocytes, and erythrocytes.
Lymphoid progenitor cells can differentiate into T-cells, B-cells and natural killer cells, which are key players in adaptive immunity.

These cells have become central in the development of cell therapies. Hematopoeitic stem cell therapies can involve autologous methods whereby the patients own stem cells are used or allogeneic, where the cells come from a donor. These treatments have been implemented in patients with certain blood cancers such as multiple myeloma and leukemia or cancers of the bone marrow.

Hematopoietic stem cells are usually derived from bone marrow, peripheral blood or umbilical cord blood. CD34 is protein marker that is used to routinely identify and isolate human hematopoietic stem/progenitor cells. In normal individuals, the percentage of CD34+ cells in the peripheral blood is very low, ranging from 0.01% to 0.1%, thus the main sources for these cell types are from bone marrow, cord blood and mobilized blood.

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CD34+ Cells
Other Hematopoietic Stem Cells

CD34+ cells

CD34+ cells are known to differentiate into all the various blood cell types. Thus, whether you are focusing on hematopoietic stem cell therapy research or for transplantation research or drug discovery, CD34+ cells can play an important role in your hematopoietic research program.

Cord blood

Cord blood-derived hematopoietic CD34+ cells tend to be more naïve, which in HLA mismatch situation will lead to fewer occurrences of host-versus-graft-disease (GVHD). This feature has made it the cell type of choice to use for development of humanized mice ( link to humanize mouse page). However, the number of CD34+cells that can be isolated from umbilical cord is a challenge due to the limiting volume of source material.
Cord Blood CD34+ cells are isolated via immunomagnetic separation and are guaranteed to have 90% purity ( product code)

Bone marrow

Bone marrow and peripheral blood-derived HSCs allow for large numbers of CD34+ cells to be harvested and transplanted, but they are of limited utility if an HLA-matched donor is unavailable due to potential allogeneic reaction. Bone marrow cells provide researchers the ability to work with large numbers of cells from a single donor or investigate differences in donors of various ages, genders, or ethnicities.

Mobilized CD34+ cells

The autologous transplantation of peripheral hematopoietic stem cells is widely used to treat solid tumors and lymphomas in children, adolescents and young adults. As In normal individuals, the percentage of CD34+ cells in the peripheral blood is very low, mobilization treatment is required to increase the number of peripheral CD34+ cells.
CD34+ hematopoietic stem and progenitor cells from mobilized blood are also used in lieu of precious patient sample for cell therapy process development and are available isolated and purified by magnetic bead isolation
CD34+ cells for cell and gene therapy development: an interview with Dr. Manon Destalminil.

CD34+ Cells for Cell and Gene Therapy Process Development

In this interview Dr. Manon Destalminil, Bioprocessing Project Leader at CellProthera, discusses the advantages of using research-grade CD34+ cells for cell and gene therapy process development. Please register to read. Download Document

Exlore our portfolio

CD34+ cells are available purified, cryopreserved and ready to use in from tissue sources such as bone marrow, cord blood or mobilized blood. In addition to cryopreserved cells, Lonza offers a range of products from fresh material for your research needs.

Cell source	Description	Format	Volume
Bone Marrow	Unprocessed bone marrow	Fresh	10 mL
	Unprocessed bone marrow	Fresh	20 mL
	CD34+ progenitor cells	Cryopreserved	≥ 0.1 mio. cells
			≥ 0.3 mio. cells
			≥ 0.5 mio. cells
			≥ 1 mio. cells
			≥ 2 mio. cells
	Mononuclear cells	Cryopreserved	≥ 5 mio. cells
			≥ 25 mio. cells

	Stromal cells	Cryopreserved	≥ 5 mio. cells

Other hematopoietic stem cells

Mononuclear cells

Mononuclear cells (MNCs) are a mixed population of single nucleus cells, such as monocytes and lymphocytes. MNCs can be further purified or pushed to differentiate into individual cell types. These cells are available from bone marrow and peripheral blood.

Mesenchymal stem cells (MSC)

MSCs are found in bone marrow and are capable of self-renewal as well as differentiation into bone, cartilage, fat, muscle, tendon, and marrow stroma cells. MSCs are excellent tools for regenerative medicine research. – Cryopreserved at passage two – Characterization: Positive for CD73, CD90, CD105, CD166 and CD44, and negative for CD14, CD19, CD34, CD45 and HLA-DR as assessed by flow cytometry. Functional testing of differentiation to osteogenic, chondrogenic, and adipogenic lineages is conducted. Optimized media kits for expansion and differentiation are available

Stromal cells

Bone marrow stromal cells are a mixed population of cell types, including fibroblasts, MSCs, adipocytes, endothelial cells, and macrophages. These cells can be used as a feeder layer for growing hematopoietic stem and progenitor cells for weeks without the need for exogenous cytokines.

Adipose-derived stem cells

Normal Human ADSC have been reported to differentiate down many different lineages including chondrogenic, osteogenic, adipogenic and neural. Normal Human ADSC have been cryopreserved at primary passage. Adipose Derived Stem Cells (hADSC) are isolated from normal (non-diabetic) adult lipoaspirates collected during elective surgical liposuction procedures.

Mesenchymal Stem Cells Infographic

Mesenchymal stem cells have extensive uses in both regenerative medicine and immunotherapy research Download Document

A 3D Bioprinted Model to Study Osteogenic Differentiation of Primary Mesenchymal Stem Cells

Learn from this white paper how a 3D bioprinted model using hMSCs can be used for evaluation of bioink compatibility and bioprinting processes. Download Document

Supporting In Vitro ADMETox with an Advanced Portfolio of Primary Cells and Media

Reference to Help Get Started on Primary Cell Culture for ADMETox Applications Download Document

Explore our portfolio

	Product	Description
Cells	hMSC - Human Mesenchymal Stem Cells	-
Media	MSCGM® Mesenchymal Stem Cell Growth Medium BulletKit®	MSCBM® Basal Medium (440 mL) + MSCGM® SingleQuots®
Media	TheraPEAK® MSCGM® Mesenchymal Stem Cell Growth Medium BulletKit®	TheraPEAK® MSCBM® Basal Medium (1 L) + supplements
Differentiation	hMSC - Human Mesenchymal Stem Cell Osteogenic Differentiation Medium BulletKit®	Osteogenic Differentiation Basal Medium (170 mL) + SingleQuots® Kit
	hMSC - Human Mesenchymal Stem Cell Chondrogenic Differentiation Medium BulletKit®	Chondrogenic Differentiation Basal Medium (185 mL) + SingleQuots® Kit
	hMSC - Human Mesenchymal Stem Cell Adipogenic Differentiation Medium BulletKit®	Adipogenic Maintenance Medium (170 mL) + SingleQuots® Kit, Adipogenic Induction Medium (170 mL) + SingleQuots® Kit