Mouse Embryonic Fibroblast Starter Nucleofector® Kit
Mouse Embryonic Fibroblast Starter Nucleofector® Kit
For determination which of the two Nucleofector® Kits is best suited for efficient transfection of a specific mouse embryonic fibroblast clone in Nucleofector® I/II/2b Device
Product Overview
The Nucleofector® 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. Due to significant diversity between different MEF clones, we have developed two Nucleofector® Kits (1 and 2) and an easy-to-use Nucleofector® Starter Kit. The Mouse Embryonic Fibroblast NucleofectorTM Starter Kit is used to determine which of the two Nucleofector® Kits is best suited for transfecting a specific primary mouse embyonic fibroblast (MEF) clone in Nucleofector® 2b System.
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
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Benefits
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low-throughput transfection in single cuvette format
Storage and Content
0.45 mL MEF 1 Nucleofector® Solution
0.45 mL MEF 2 Nucleofector® Solution
2 x 0.1 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
10 Aluminum cuvette (100 µL)
10 Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
