For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-Nucleofector® X Unit (20 µL format).
Basal media required for growth of Endothelial cells, 500 mL (supplements and growth factors sold separately)
Cryopreserved ampule of Adult Human Dermal Microvascular Endothelial Cells (HMVEC-d Ad) containing ≥ 500,000 cells
Culture system containing EBM® Basal Medium (CC-3121) and EGM® Endothelial Cell Growth Medium SingleQuots® Supplements (CC-4133) required for growth of endothelial cells
EGM®-Plus Endothelial SingleQuot® Kit
Cryopreserved ampule of Normal Human Pulmonary Artery Endothelial Cells (HPAEC) containing ≥ 500,000 cells
For efficient transfection of primary human DCs (dendritic cells) in the Nucleofector® I/II/2b Device
For efficient transfection of primary mouse B cells in the Nucleofector® I/II/2b Device
For efficient transfection of primary mouse T cells in the Nucleofector® I/II/2b Device
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Instructions - UltraCHO™ MediumHow to use ProCHO medium
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Instructions – ProCHO Liquid mediaInstructions for use of ProCHO Media
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Instructions ProCHO powder kitsInstructions for use of ProCHO Powder kits
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ProCHO® Protein Expression Media
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WinKQCL™ 5 Remote Client Installation InstructionsWinKQCL™ 5 client installation system requirements and configuration.
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WinKQCL 5 Software - EnglishWe Analyze Endotoxin Data Every Day Version 5 Software Features Announcement of discontinued support for Windows SQL Server® 2000 and XP Operating System English Version
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WinKQCL™ 5 Endotoxin Detection SoftwareLearn how to use the advanced features in Lonza's WinKQCL™ Endotoxin Detection Software to save time, reduce costs and ensure compliance to 21 CFR Part 11 and Annex 11. -
BE02-056Q CHO Xtreme™ Feed CD - InstructionsBE02-056Q
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4D-Nucleofector® Protocol for MRC-5Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MRC-5
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MRC-5 Transfection Protocol - 4D-Nucleofector™ DeviceInstructions of use for transfection of MRC-5 with the SE Cell Line 4D-Nucleofector™ X Kit