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EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™

EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™

Catalog #: CC-3162

Culture system containing EBMTM-2 Basal Medium (CC-3156) and EGMTM-2 SingleQuotsTM Supplements (CC-4176) required for growth of Endothelial Cells. Available for immediate shipment.

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234.00 EUR
SA

Product Overview

Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. EGMTM - 2 Medium is suitable to grow most types of large vessel endothelial cells. Each type of endothelial cell growth medium is guaranteed to perform when used to grow Lonza's Endothelial Primary Cells.

The following Endothelial Cell Growth Medium BulletKitsTM are available: 

  • EGMTM BulletKitTM (CC-3124) – Optimized for HUVECs cat. no. CC-2517 and CC-2519. Contains BBE . Final concentration of FBS in the mixed BulletKit is 2%. Does not contain exogenous VEGF.
  • EGMTM-Plus BulletKitTM (CC-5035) – Enhanced version of EGMTM Medium. Optimized for HUVECs cat. no. CC-2935. Contains BBE. Final concentration of FBS in the mixed BulletKit is  2%. Does not contain exogenous VEGF. Faster proliferation than HUVECs isolated in EGMTM Medium.
  • EGMTM-2 BulletKitTM (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Final concentration of FBS in the mixed BulletKit is  2%.  Contains VEGF for rapid proliferation. Does not contain BBE.
  • EGMTM-2 MV BulletKitTM (CC-3202) – Coronary and Iliac artery, human microvascular endothelial cells. Final concentration of FBS in the mixed BulletKit is 5%.  Contains VEGF for rapid proliferation. Does not contain BBE.

Lonza guarantees the performance of endothelial cells only if appropriate EGM Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

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Storage and Content

1 x EBMTM-2 Basal Medium (CC-3156), 500 mL

1 x EGMTM-2 SingleQuotsTM Supplement Pack (CC-4176) containing:

1 x Bottle FBS, 10.00 mL
1 x Natural Cap Vial with Hydrocortisone, 0.20 mL
1 x Gray Cap Vial with hFGF-B, 2.00 mL
1 x White Cap Vial with VEGF, 0.50 mL
1 x Yellow Cap Vial with R3-IGF-1, 0.50 mL
1 x Blue Cap Vial with Ascorbic Acid, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Orange Cap Vial with Heparin, 0.50 mL

Download our Endothelial Cell Growth Medium Concentration Guide

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • TechSheet - Pulmonary Artery Endothelial Cell Systems

    Technical information sheet for Clonetics™ Pulmonary Artery Endothelial Cells
  • TechSheet – Endothelial Cell System Medium Products

    Technical information sheet for Clonetics™ Endothelial Cell Medium Products
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • Instructions & Technical Info - Endothelial Cell Systems

    Technical Information and Instructions for Proper Use of Endothelial Cells
  • Instructions for Use - HUVEC Culturing and Angiogenesis Inhibition Assay using Sartorius Incucyte®

    Protocol for Angiogenesis assay using HUVEC cells
  • Primary Human Umbilical Vein Endothelial Cells

    Tech note demonstrating the successful development of tube formation assay using primary HUVECs that can be used to visualize angiogenesis in real-time.
  • Human Umbilical Vein Endothelial Cell and Normal human Dermal Fibroblast Co-Culture and Angiogenesis Assay

    CD-OP026_HUVEC Fibroblast Protocol.pdf
  • A Quantitative, High-throughput, Real-time Angiogenic Tube Formation Assay

    Angiogenic Assay - Human Umbilical Vein Endothelial Cells and Human Dermal Fibroblasts in Co-culture
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Educational Material

Brochures, White Papers etc.

  • Poster - AACR 2017

    Simple and easy monitoring of tube formation and migration assays with the CytoSMART™ Live Cell Imaging System
  • White Paper

    Simple and easy monitoring of three different cancer-relevant assays – Tube formation assay, 2D cell migration assay and 3D cell invasion assay
  • Lonza's Bulletkit™ for Primary Cell Growth

    Primary Cells are Different from Cell Lines. Why use the Same Classical Media? Learn how Lonza primary cells and media can support your cell research
  • Assessment of the Anti-angiogenic Effect of VEGFR2 siRNA in HUVEC

    Scientific poster for AACR 2015
  • Diabetes-related Differential Gene Expression in HAECs and ADSCs

    With the prevalence of diabetes growing worldwide, the availability of primary human cells from diabetic donors is critical to increase research and knowledge about the disease at a cellular level. In this study, we sought to identify genes differentially regulated in diabetic type 1 and type 2 adipose-derived stem cells (ADSCs) and human aortic endothelial cells (HAECs).
  • Primary Human Airway Cells and Media

    Airway Cell and Media Reference Guide
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • Primary Cell Types Provide a Ready-to-Use Platform for Cell-Based Assays

    Poster presented at Miptec, Basel (CH) - Sep 21-24, 2010
  • First siRNA Library Screening in Hard-to-Transfect HUVEC and Jurkat Cells

    High throughput transfection of siRNA libraries has become a valuable tool in target identifi cation and validation. However, such screenings have so far been constrained to mostly easy-totransfect adherent cell lines. Lonza’s 96-well Shuttle® System, based on the well-established Amaxa® Nucleofector® Technology, extends these approaches to primary and diffi cult-to-transfect cells.
  • Primary Cells for Airway Research – Infographic

    Infographic showing how Lonza primary cells can aide in your airway research
View More
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Updated Angiogenesis Assay

Access our latest angiogenesis technical note and protocol for use with our human umbilical vein endothelial cells (HUVEC) in EGMTM-2 BulletKitTM Medium.

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