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NK - Human Natural Killer Cells, Cryopreserved, Negative Selection, 5 Million
Catalog #: 2W-501
Crypreserved ampule of Peripheral Blood Human Natural Killer Cells (CD56 negative immunomagnetic selection) containing ≥ 5 million cells
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Unprocessed Human Bone Marrow, Fresh, 25 mL
Catalog #: 1M-125
Fresh Unprocessed Human Bone Marrow, 25 mL
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SeaKem® LE Agarose
Catalog #: 50002
SeaKem® LE Agarose, 100 g, ideal for routine analysis of nucleic acids by gel electrophoresis and blotting.
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X-VIVO® 10 Serum-free Hematopoietic Cell Medium
Catalog #: 04-380Q
X-VIVO® 10, serum-free hematopoietic cell medium, with L-Glutamine, gentamicin and phenol red, xenofree, 1 L
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Insect-XPRESS™ Protein-free Insect Cell Medium
Catalog #: 12-730Q
Insect-XPRESSTM Protein-free Insect Cell Medium with L-Glutamine, 1L
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NuSieve® GTG® Agarose
Catalog #: 50081
NuSieve® GTG® Agarose, 25 g, fine resolution of small DNA and certified for enzymatic reactions.
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Human Bone Marrow CD34+ Progenitor Cells, Cryopreserved, 1 Million
Catalog #: 2M-101C
Bone Marrow CD34+ Cells, 1 Million, Cryopreserved, Positively Selected
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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