Assay designed to measure toxicity in mammalian cells and cell lines in culture
Cryopreserved ampule of Human Umbilical Vein Endothelial Cells (HUVEC) without VEGF, from a single donor, cultured in EGMTM Plus and containing ≥ 500,000 cells
Cryopreserved ampoule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells
Cryopreserved ampoule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 25 million cells
Cryopreserved ampoule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 10 million cells
Supplements and growth factors required for culture of endothelial cells (basal media sold separately)
Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 50 million cells with cell population frequency information
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Troubleshooting Guide - PYROGENT® Gel Clot LAL AssayMost problems encountered in the use of the PYROGENT® Gel Clot Limulus Amebocyte Lysate (LAL) Assay fall into two basic categories - no firm gels are observed in reaction tubes where firm gels are expected or firm gels form in reaction tubes, which are expected to yield negative results. This Tech Tip takes a deeper look into each of these categories and provides troubleshooting guidance.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (English)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (French)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Japanese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Portuguese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Spanish)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Italian)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Chinese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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How To Perform The PYROGENTâ„¢ Gel Clot LAL AssayThe gel clot LAL assay is a qualitative test that provides simple positive-negative results. This video demonstrates how to perform the assay from start to finish. -
Instructions - PYROGENTâ„¢ and PYROGENTâ„¢ Plus (Chinese)Detailed instructions for performing the PYROGENTâ„¢ and PYROGENTâ„¢ Plus Gel Clot LAL Assay