Product Overview
Gel Clot LAL (PYROGENTTM) provides a simple positive/negative result and is most often mentioned in pharmacopeial monographs as the official referee test. Lonza’s PYROGENTTM Gel Clot LAL Assay is a qualitative LAL test for Gram-negative bacterial endotoxin. The gel clot assay is run in tubes that are placed in a water bath or dry heat block at 37°C. After a one-hour incubation period, the tubes are flipped 180°. A firm clot that stays in the bottom of the tube indicates a positive reaction. If liquid flows down the side of the tube, the result is negative for endotoxin. PYROGENTTM Gel Clot LAL Assay requires depyrogenated 10 x 75 mm glass reaction tubes to run the assay.
Request More InfoBenefits
Sensitivity of 0.06 EU/ml Easy-to-read qualitative results
Applications
Simple LAL test not requiring sophisticated instrumentation and software
Storage and Content
5 x 50 tests/vial lysateSDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Instructions - PYROGENT® and PYROGENT® Plus (English)
Detailed instructions for performing the PYROGENT® and PYROGENT® Plus Gel Clot LAL Assay
Educational Material
Brochures, White Papers etc.
Videos
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Technical Tips - PYROGENT® Gel Clot
QC testing professionals and researchers sometimes encounter issues when performing the gel clot LAL test. This Tech Tip will explore ways to help overcome these challenges including the use of appropriate accessory items, correct incubation method and proper technique for flipping the tubes. -
Troubleshooting Guide - PYROGENT® Gel Clot LAL Assay
Most problems encountered in the use of the PYROGENT® Gel Clot Limulus Amebocyte Lysate (LAL) Assay fall into two basic categories - no firm gels are observed in reaction tubes where firm gels are expected or firm gels form in reaction tubes, which are expected to yield negative results. This Tech Tip takes a deeper look into each of these categories and provides troubleshooting guidance. -
Instructions - PYROGENT® and PYROGENT® Plus (English)
Detailed instructions for performing the PYROGENT® and PYROGENT® Plus Gel Clot LAL Assay -
Troubleshooting Guide - Testing Oil Solutions with PYROGENT™ Gel Clot LAL Assay
Endotoxins, due to their lipid character, tend to remain associated with oils and do not readily enter the aqueous phase of water-oil mixtures. This Tech Tip describes the use of a dispersing agent, PYROSPERSE™, to allow for dissociation of the endotoxin from the oils and for subsequent detection of endotoxin in the aqueous phase by the PYROGENT™ Gel Clot LAL Assay. -
Troubleshooting Guide - Gel Clot LAL Assay Procedure (English)
A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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How To Perform The PYROGENT™ Gel Clot LAL Assay
The gel clot LAL assay is a qualitative test that provides simple positive-negative results. This video demonstrates how to perform the assay from start to finish.