SF Cell Line 96-well Nucleofector® Kit
SF Cell Line 96-well Nucleofector® Kit
For medium-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 4D-Nucleofector® 96-well Unit
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Product Overview
The SF Cell Line 96-well Nucleofector® Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 4D-Nucleofector® 96-well Unit.
This kit has been determined to be optimal for transfecting following cell types:
- 293-F (FreeStyle), 32D
- A-20, A2058, A375, A431, A549, AGS, ARPE-19
- BetaTC/6, BxPC-3
- C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
- EL4
- FDC-P1
- H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
- IMR-32
- J774A.1
- K-562
- KG-1
- L-428, LNCaP
- MDA-MB-453, MDBK, Molt-4, MV-4-11
- Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
- P19, P815, PC-12, PC-3
- Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
- S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
- T2, T84, TF-1
- U266B1, U-937
- Vero
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.
Request More InfoBenefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Compatible with liquid handling systems for automated high-throughput electroporation
- Conditions are transferable between 4D-Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System
Applications
- Transfection of low cell numbers in the range of 5 x 104 to 1 × 106 cells
- High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Storage and Content
2.25 mL SF Cell Line Nucleofector® Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 96-well Nucleocuvette® Plate (20 μL)
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
