Kinetic-QCL™ Bulk Kinetic Chromogenic LAL Assay, 600 Test Kit
Kinetic-QCL™ Bulk Kinetic Chromogenic LAL Assay, 600 Test Kit
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Product Overview
This is a bulk configuration of the KQCL(TM) Kinetic Chromogenic LAL Endotoxin Detection Assay and is available as a made-to-order product. This bulk kit configuration does not contain endotoxin and requires that E50-643L is ordered separately. Testing of specific lots for making larger product reserves is possible. Please contact your local sales representative for further details. The Kinetic-QCLTM Kinetic Chromogenic LAL Assay is a quantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent in a 96-well plate and placed in an incubating plate reader that measures absorbance at 405 nm. The reaction is automatically monitored over time for the appearance of a yellow color.
In the presence of endotoxin, the lysate will begin to cleave the chromogenic substrate, causing the solution to become yellow. The time required for the change is inversely proportional to the amount of endotoxin present. The concentration of unknown samples can then be calculated from a standard curve. It is the chromogenic nature of the Kinetic-QCLTM Kinetic Chromogenic LAL Assay that makes it the most appropriate choice for testing small volume parenterals, vaccines, antibiotics and biologicals. The Kinetic-QCLTM Assay is less impacted by inhibitory products which may interfere with the clotting mechanism in turbidimetric and gel clot assays. With a sensitivity range of 0.005 EU/ml - 50.0 EU/ml, the Kinetic-QCLTM Kinetic Chromogenic LAL Assay is the most sensitive of the LAL assays.
Benefits
Most sensitive LAL-based method Less sensitive to product inhibition
Applications
Ideal for biological products such as vaccines and antibiotics
Storage and Content
25 x 24 tests/vial LAL LysateSDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Instructions - Limulus Amebocyte Lysate (LAL) Kinetic-QCL® (English)
Detailed instructions for performing the Kinetic-QCL® Kinetic Chromogenic LAL Assay in English -
Troubleshooting Guide - Kinetic LAL Assay Procedure (English)
A step-by-step guide depicting how to perform a traditional kinetic LAL assay.
Educational Material
Brochures, White Papers etc.
Videos
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Troubleshooting Guide - Testing Oil Solutions with the PYROGENT™-5000 and Kinetic-QCL™ Assays
Oil-based parenterals have always been a challenge in endotoxin detection assays. This Tech Tip describes a liquid-liquid extraction procedure that can be used to remove the endotoxin from the oil into an aqueous phase, which can then be tested with the PYROGENT™-5000 or Kinetic-QCL™ Assays. -
Instructions - Limulus Amebocyte Lysate (LAL) Kinetic-QCL® (English)
Detailed instructions for performing the Kinetic-QCL® Kinetic Chromogenic LAL Assay in English -
Troubleshooting Guide - Kinetic LAL Assay Procedure (English)
A step-by-step guide depicting how to perform a traditional kinetic LAL assay. -
Technical Tip - Serum, Plasma Testing with PYROGENT® 5000 and Kinetic-QCL® Assays
The article focuses on the recommended procedure that can be used to prepare serum or plasma for LAL-based endotoxin testing.
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How To Perform The Kinetic-QCL™ LAL Assay
The Kinetic-QCL™ Kinetic Chromogenic LAL Assay is a quantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. This video features three system components: reagents, software and an absorbance plate reader and demonstrates how to perform an inhibition/enhancement assay.