text.skipToContent text.skipToNavigation
Need help? Please contact us

PDELight™ HTS cAMP Phosphodiesterase Assay Kit, 500 Test

PDELight™ HTS cAMP Phosphodiesterase Assay Kit, 500 Test

Catalog #: LT07-600

PDELightTM Assay Kit is a generic, homogeneous assay designed for use in high throughput screening (HTS) of phosphodiesterase activity, 500 Tests

Compare

 465.00 CHF
EA

Product Overview

The PDELightTM Assay Kit is a generic, homogeneous assay designed for use in high throughput screening of phosphodiesterase activity. It uses the power of bioluminescent detection to provide a simple alternative to existing phosphodiesterase assays with a generic endpoint determination for use with all cAMP dependant phosphodiesterases.

The AMP produced from phosphodiesterase hydrolysis of cAMP is quantified using a robust and highly sensitive luciferase-based luminescent reagent. The AMP is directly converted to ATP and quantified as light. Nearly a photon of light is emitted for every molecule of ATP produced.

The assay can easily be optimized for each phophodiesterase to produce rapid, quality data suitable for IC50 determinations of screen compounds. The signal is glow luminescence with a half-life of greater than two hours, which is detected using a luminometer. The assay is extremely simple to use and can be run in several protocols to suit your needs.

 

Request More Info

Benefits

  • Simple, Homogeneous Assay - Add reagents directly to the completed PDE reaction and read.
  • Generic Platform - Use the same assay for all cAMP dependent phosphodisesterases. Eliminate antibodies, radioactive beads, or modified substrates.
  • Non-radioactive - Non-hazardous reagents, no costly disposal costs.
  • Rapid Assay - Complete a 384-well plate in less than 15 minutes.
  • Bioluminescent Sensitivity - Use small amounts of enzyme in 96-, 384-, or 1536-well formats.
  • Reproducible and Robust - Low false positive rates, typical Z´values > 0.7, and few artifacts result in good clean hits.

Storage and Content

1 x LT27-250 PDELightTM AMP Detection Reagent, 10 mL
1 x LT27-253 PDELightTM Stop Solution, 10 mL
1 x LT27-256 PDELightTM Reconstitution Buffer, 20 mL

Return to Top

SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Instructions – PDELight™ HTS cAMP Phosphodiesterase Kit

    Instructions for proper use of PDELight™ cAMP Phosphodiesterase HTS Assay Kit
Return to Top

Subscribe to our eNewsletter

Keep up to speed on the latest scientific developments, events, tips and tools from Lonza.

Interested in our webinars?

Need a break from your daily routine? Grab a coffee and check out our free webinars.