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Next-generation large-scale electroporation

Expedite your path from research to GMP manufacturing with our next-generation large-scale electroporation platform, the 4D-Nucleofector® LV Unit PRO, optimized for the delivery of clinically relevant cargos.

Get in touch with our Nucleofection® Experts to learn more about our next-generation large-scale electroporation.

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Why 4D-Nucleofector® LV Unit PRO? Key benefits:

Improved scalability

Easily scale up to 1 billion cells for CRISPR-based knock-out/in or transposition

High flexibility

Fixed volume cartridge suited for volumes between 0.5 and 2 mL

GMP compatible

GMP consumables, 21CFR part 11 compliant software, IQOQ services

See the science:
performance data

Proven performance for different cargo types, T cell sources, donors, volumes, cell concentrations and target loci

Scientific poster

Learn about the scale up for CRISPR knock-in and knock-out

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Webinar

Dive deep into CRISPR and transposon data

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Why are your peers
using our large-scale
electroporation platform?

Research and GMP consumables, 21CFR part 11 compliant software, IQOQ services and more

Seamless translation from research to GMP manufacturing

First generation LV Unit used in more than 10 clinical trials

Established path to the clinic

Based on proven Nucleofector® Technology

Scale up your trusted and reliable non-viral transfection method

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Get details and personalized advice from one of our transfection specialists.

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Questions? We've got answers

Find answers to commonly asked questions about our 4D-Nucleofector® LV Unit PRO

Is the 4D-Nucleofector® LV Unit PRO a new technology?

It is the second generation of our existing large-scale electroporation platform, 4D-Nucleofector® LV Unit which is in the market for almost a decade and already used in GMP manufacturing for clinical trials. The new generation provides improved scale-up performance for more complex cargo situations like CRISPR-based knock-in or transposon-based integration.

How many cells can be transfected with the 4D-Nucleofector® LV Unit PRO?

With the fixed volume cartridge you can scale up to 100 - 200 million cells, with the flow-through you can easily scale up to 1 billion cells.

How do I do the scale up from the small-scale 4D-Nucleofector® X Unit to the 4D-Nucleofector® LV Unit PRO?

Depending on the cell-cargo combination used, a selection of 2 - 3 large-scale programs has to be tested using 500 µL in the fixed volume cartridge. We are providing specific recommendations for that approach. Further fine-tuning can be done if needed. The identified program can then be used for other volumes in the fixed volume cartridge or for the flow-through cartridge.

Can I use the next-generation cartridges with my existing 4D-Nucleofector® LV Unit?

An upgrade to the 4D-Nucleofector® LV Unit PRO is necessary and will allow you to use the next-generation cartridges. Please fill out the form or contact your local sales rep for upgrade conditions.