Product Overview
The SG Cell Line 4D-Nucleofector® X Kit S is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette® Strip format when working with the 4D-Nucleofector® X Unit.
This kit has been determined to be optimal for transfecting following cell types:
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection® conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Request More InfoBenefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
Applications
- Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
- Medium throughput applications
- Optimization of transfection conditions, e.g. substrate titrations
Storage and Content
1 x 0.675 mL SG Cell Line Nucleofector® Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μl)
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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4D-Nucleofector® Protocol for Ramos
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Ramos -
4D-Nucleofector® Protocol for THP-1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for THP-1 -
4D-Nucleofector® Protocol for H4
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for H4 -
4D-Nucleofector® Protocol for Colo-205
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Colo-205 -
4D-Nucleofector® Protocol for BAF3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for BAF3 -
4D-Nucleofector® Protocol for Raji
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Raji -
4D-Nucleofector® Protocol for WEHI-231
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for WEHI-231 -
4D-Nucleofector® Protocol for TD1 ORL UVA
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for TD1 ORL UVA -
4D-Nucleofector® Protocol for NIH3T3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for NIH3T3
Educational Material
Videos
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How it Works - 4D-Nucleofector® System (Video Tutorial)
Workflow overview for using the 4D-Nucleofector™ System -
How it Works - 4D-Nucleofector™ X Unit (Video Tutorial)
Workflow overview for using the 4D-Nucleofector™ X Unit
