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Cell Line Nucleofector® Kit RCatalog #: VCA-1001
For efficient transfection of cell lines, e.g. HeLa, IMR-90, NIH/3T3, NK-92, or Sf9, in the Nucleofector® I/II/2b Device
Sales unit: items -
SE Cell Line 96-well Nucleofector® KitCatalog #: V4SC-1096
For medium-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 4D-Nucleofector® 96-well Unit
Sales unit: kit -
SE Cell Line 96-well Nucleofector® Kit (960 RCT)Catalog #: V4SC-1960
For medium-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 4D-Nucleofector® 96-well Unit
Sales unit: kit -
SE Cell Line 384-well Nucleofector® KitCatalog #: V5SC-1002
For high-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 384-well HT Nucleofector® System.
Sales unit: kit -
SE Cell Line 384-well Nucleofector® KitCatalog #: V5SC-1010
For high-throughput transfection of cell lines, e.g. Jurkat or MCF-7, when working with the 384-well HT Nucleofector® System.
Sales unit: items -
SE Cell Line 4D-Nucleofector® X Kit SCatalog #: V4XC-1032
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (20 µL format).
Sales unit: kit -
SE Cell Line 4D-Nucleofector® X Kit LCatalog #: V4XC-1024
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (100 µL format).
Sales unit: kit -
SE Cell Line 4D-Nucleofector® X Kit LCatalog #: V4XC-1012
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (100 µL format).
Sales unit: kit -
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Hanks' Buffered Saline Solution, HBSS without calcium and magnesium, with phenol redCatalog #: 10-543F
Hanks' BSS (1X), HBSS without calcium and magnesium, with phenol red, 500mL.
Please note: this product is no longer available in Europe and Africa.
For customers in the Americas and Asia-Pacific regions, the product will be discontinued by June 30, 2022. It will be sold until stock is depleted.
Sales unit: Bottle
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit