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P3 Primary Cell 384-well Nucleofector® KitCatalog #: V5SP-3010For high-throughput transfection of primary cells, e.g. human T cells, DCs or CD34, when working with the 384-well Nucleofector® System.Sales unit: items
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Cryo Human Leukopak (5.0 Billion Cells)Catalog #: 260260.01
Cryopreserved human leukapheresis product (≥5.0 billion cells)
Sales unit: Bag -
Cryo Human Leukopak (9.5 Billion Cells)Catalog #: 260240.01
Cryopreserved human leukapheresis product (≥9.5 billion cells)
Sales unit: Bag -
P3 Primary Cell 96-well Nucleofector® Kit (960 RCT)Catalog #: V4SP-3960For medium-throughput transfection of primary cells, e.g. human T cells, CD34+ or rat neurons, when working with the 4D-Nucleofector® 96-well Unit.Sales unit: kit
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hADSC Human Adipose-Derived Stem Cells, 1 MillionCatalog #: PT-5006
Cryopreserved ampule of Human Adipose-Derived Stem Cells (hADSC) containing ≥1 million cells
Sales unit: Ampule -
Cryo Human Leukopak (2.5 Billion Cells)Catalog #: 260280.01Cryopreserved human leukapheresis product collected from normal peripheral blood, containing ≥2.5 billion cellsSales unit: Bag
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Fresh Human Leukopak (5 Billion Cells)Catalog #: 3P-105BFresh human leukopheresis product collected from normal peripheral blood, containing ≥ 5 billion cellsSales unit: each
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Fresh Human Leukopak (10 Billion Cells)Catalog #: 3P-105Fresh human leukapheresis product collected from normal peripheral blood, containing ≥ 10 billion cellsSales unit: each
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P3 Primary Cell 4D-Nucleofector® X Kit LCatalog #: V4XP-3024
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (100 µL format).
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NHDC - Human Dendritic Cells, 2.5 MillionCatalog #: CC-2701
Cryopreserved ampule of Normal Human Dendritic Cells (NHDC) containing ≥2.5 million cells
Sales unit: Ampule
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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