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NK - Human Natural Killer Cells, Cryopreserved, Negative Selection, 5 MillionCatalog #: 2W-501
Crypreserved ampule of Peripheral Blood Human Natural Killer Cells (CD56 negative immunomagnetic selection) containing ≥ 5 million cells
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DPSC - Human Dental Pulp Stem CellsCatalog #: PT-5025
Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells
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Human Bone Marrow MNCs, Cryopreserved, 5 MillionCatalog #: 2S-101D
Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing ≥5 million cells
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NPMMTM Neural Progenitor Maintenance Medium BulletKit®Catalog #: CC-3209
Culture system containing PBMTM Neural Progenitor Basal Medium (CC-3210), NPMMTMMaintenance Media SingleQuots® (CC-4241) and Neural Progenitor Supplement SingleQuots® (CC-4242) required for growth and differentiation of neural progenitor cells
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Fresh Human Bone Marrow MNCs, 100MCatalog #: 1M-125DFresh Bone Marrow Mononuclear Cells, 100 million cellsSales unit: each
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P3 Primary Cell 4D-Nucleofector® LV Kit LCatalog #: V4LP-3002For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV UnitSales unit: kit
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Human Bone Marrow Mononuclear Cells MNCs, Cryopreserved, 25 Million CellsCatalog #: 2M-125C
Cryopreserved ampule of Human Bone Marrow Mononuclear Cells containing ≥25 million cells
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Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 MillionCatalog #: 2W-200Cryopreserved ampule of Human Peripheral Blood CD4+ T Cells containing ≥10 million cells, negatively selectedSales unit: each
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P3 Primary Cell 4D-Nucleofector® LV Kit XL (5 x 1 RCT)Catalog #: V4LP-3520For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV UnitSales unit: kit
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P3 Primary Cell 4D-Nucleofector® LV Kit XLCatalog #: V4LP-3020For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV UnitSales unit: kit
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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