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Human CD34+ Cell Nucleofector® KitCatalog #: VPA-1003
For efficient transfection of primary human CD34+ cells in the Nucleofector® I/II/2b Device
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P3 Primary Cell 4D-Nucleofector® LV Kit LCatalog #: V4LP-3002For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV UnitSales unit: kit
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Fresh Human Leukopak - Neupogen® Mobilized (Day 5 Collection, Full Bag)Catalog #: 3Y-300Fresh human leukopheresis product collected from peripheral blood mobilized with Neupogen(R) 10 mcg/kg/day x 5 days, 1 Bag, Day 5 CollectionSales unit: each
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Fresh Human Leukopak - Neupogen(R) Mobilized (Day 6 Collection, Full Bag)Catalog #: 3Y-300BFresh human leukopheresis product collected from peripheral blood mobilized with Neupogen(R) 10 mcg/kg/day x 5 days, Second Collection, Day 6, 1 BagSales unit: each
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Human Peripheral Blood CD4+ T Cells, Cryopreserved, 10 MillionCatalog #: 2W-200Cryopreserved ampule of Human Peripheral Blood CD4+ T Cells containing ≥10 million cells, negatively selectedSales unit: Ampule
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P3 Primary Cell 4D-Nucleofector® LV Kit XL (5 x 1 RCT)Catalog #: V4LP-3520For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV UnitSales unit: kit
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P3 Primary Cell 4D-Nucleofector® LV Kit XLCatalog #: V4LP-3020For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV UnitSales unit: kit
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DPSC - Human Dental Pulp Stem CellsCatalog #: PT-5025
Cryopreserved ampule of Human Dental Pulp Stem Cells containing ≥1 million cells
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P3 Primary Cell 96-well Nucleofector® Kit (960 RCT)Catalog #: V4SP-3960For medium-throughput transfection of primary cells, e.g. human T cells, CD34+ or rat neurons, when working with the 4D-Nucleofector® 96-well Unit.Sales unit: kit
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P3 Primary Cell 384-well Nucleofector® KitCatalog #: V5SP-3002For high-throughput transfection of primary cells, e.g. human T cells, DCs or CD34, when working with the 384-well Nucleofector® System.Sales unit: kit
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Terms and ConditionsCD34+ cells BOGO
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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CD34+ Cells for Cell and Gene Therapy Process DevelopmentIn this interview Dr. Manon Destalminil, Bioprocessing Project Leader at CellProthera, discusses the advantages of using research-grade CD34+ cells for cell and gene therapy process development. Please register to read.
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 4D-Nucleofector™ X Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of human CD34+ cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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