For high-throughput transfection of primary cells, e.g. human ADSC or MSC, when working with the 384-well HT Nucleofector® System.
For medium-throughput transfection of cell lines, e.g. Ba/F3, Raji or THP-1, when working with the 4D-Nucleofector® 96-well Unit
For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-Nucleofector® X Unit (20 µL format).
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For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-Nucleofector® X Unit (20 µL format).
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-Nucleofector® X Unit (20 µL format).
For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-Nucleofector® X Unit (100 µL format).
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-Nucleofector® X Unit (100 µL format).
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References - X-Vivo™Overview of relevant references
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X-VIVO Reference Guide 2025A curated list of peer-reviewed articles related to X-VIVO RUO media as used for culturing different primary cells and is a resource for potential consumers to find scientific data.
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TheraPEAK® X-VIVO® Hematopoietic Cell Culture MediumSerum-free, GMP produced medium for cell therapy applications
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Instructions for Use – TheraPEAK® X-VIVO® Cell Culture MediumInstructions for how to use the X-VIVO® Media Series
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Developing Patient-specific Cell Therapy Manufacturing ProcessesPoster presentation about patient-specific cellular therapies and their tremendous potential for being able to treat life threatening diseases where there are currently no cures.
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Serum-free Media FFM Part Codes2019 change of all RUO Media to FFM production overview of RUO and new FFM part codes (X-VIVO not included)
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Terms and Conditions TheraPEAK® T-VIVO® Sample Request ProgramTerms and Conditions TheraPEAK® T-VIVO® Sample Requests
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Primary Normal Human Cells and MediaIn Vivo Relevance. In Vitro Results. Updated and revised cell content.
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Non-viral Gene Modification for Ex-vivo Cell TherapyIn this interview our Director Transfection R&D discusses the advantages of non-viral transfection technologies for CRISPR- or transposon-based genetic modification cell and gene therapy.
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TheraPEAK® T-VIVO® Cell Culture Medium – OverviewView or download this document for a comprehensive overview and learn how the chemically defined TheraPEAK® T-VIVO® Medium enhances T-cell culture and accelerates therapy development.