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P3 Primary Cell 4D-Nucleofector® X Kit L
Catalog #: V4XP-3012
For efficient transfection of specific primary cells, e.g. human T cells, CD34 or rat neurons, in the 4D-Nucleofector® X Unit (100 µL format).
NPMMTM Neural Progenitor Differentiation Maintenance Medium SingleQuots® Supplements
Catalog #: CC-4241
Neural Progenitor Maintenance Medium
Human Naïve CD8+/CD45RA+ T Cells, 5 Million
Catalog #: 4W-302
Human CD8+/CD45RA+ naïve cytotoxic T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 5 million cells, cryopreserved
Human Naïve CD8+/CD45RA+ T Cells, 5 Million
Catalog #: 2W-302
Human CD8+/CD45RA+ naïve cytotoxic T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 5 million cells, cryopreserved
HMVEC-C – Human Cardiac Microvascular Endothelial Cells
Catalog #: CC-7030
Cryopreserved ampule of Human Cardiac Microvascular Endothelial Cells (HMVEC-C) containing ≥ 500,000 cells
Human CD4+/CD45RA+ Naive T Cells, 5 Million
Catalog #: 4W-202
Human CD4+/CD45RA+ Naive T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 5 million cells, cryopreserved
Human CD4+/CD45RA+ Naïve T Cells, 5 Million
Catalog #: 2W-202
Human CD4+/CD45RA+ Naïve T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 5 million cells, cryopreserved
Liver-Derived Endothelial Cells, human, passage 2
Catalog #: HLECP2
Human Liver-Derived Endothelial Cells
Note: Product will be discontinued follow depletion of current inventory. HLECP1 is the equivalent replacement.
Human CD4+/CD45RO+ Memory T Cells, 5 Million
Catalog #: 4W-204
Human CD4+/CD45RA+ memory T cells, isolated from peripheral blood using negative immunomagnetic selection, ≥ 5 million cells, cryopreserved
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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