Instructions of cell culture plate coating procedure required for successful neuronal culture

Supplemental instructions for suggested cryopreservation protocol for CloneticsTM and PoieticsTM primary cells

Supplemental instruction sheet for Clonetics^® ReagentPack™ Subculture Reagents

Supplemental myotube differentiation instructions for ClonticsTM Human Skeletal Muscle Myoblasts

This guide provides expert guidance on fundamental cell culture techniques plus safety and troubleshooting tips.

Drug-Induced Liver Injury (DILI) toxicology modeling throughout the discovery pipeline

Time-Lapse Video of Human Umbilical Vein Endothelial Cell Angiogenic Tube Formation in Co-Culture with Dermal Fibroblasts. Primary Human Umbilical Vein Endothelial Cells (HUVECs) were co-cultured with primary Human Normal Dermal Fibroblast cells at a ratio of 1:3 HUVECs: fibroblasts and a total cell density of 40,000 cells/well in a 96-well plate. Co-cultures were maintained for 5 days with medium changes every 2-3 days in EBMTM-2 medium supplemented with a custom concentration of VEGF (313 pM) to optimize angiogenic tube formation in this setting. Cells were stained with CellTracker^® Dye from ThermoFisher. Purple lines indicate angiogenic tube formation detected by the live cell imaging software. At each frame, the software quantifies angiogenesis by counting the number of network branch points (i.e., the number of branches in each network of tubes) per mm2 and the mean network length (i.e., the average of the total network length of tubes per mm2).

In this podcast, our cell culture media experts explore growing cells for therapeutic purposes. What is cell culture media, what does it take to grow cells in a lab and which future trends does the industry need to address?

Technical tips for culturing Rat Astrocytes